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Abstract
Purpose
The purpose of this study was to quantify and compare the expressions of CRP and M-CSF in the gingival tissues of the patients with chronic periodontitis associated to hypertension.
Methods
Gingival tissue samples were obtained during periodontal surgery or tooth extraction. Clinically healthy gingival tissue samples from systemically healthy 12 patients were categorized as group 1 (n=12). Inflammatory gingival tissue samples from patients with chronic periodontitis were categorized as group 2 (n=12). Inflammatory gingival tissue samples from patients with chronic periodontitis associated with hypertension were categorized as group 3 (n=12). Tissue samples were prepared and analyzed by Western blotting. The quantification of CRP and M-CSF were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test.
Results
There were significant differences between group 1 and group 2 and between group 1 and group 3 in both CRP and M-CSF. The differences between group 2 and group 3 were not statistically significant in both proteins. However, the expression levels of CRP and M-CSF in hypertensive inflammatory gingiva showed increased tendency compared to non-hypertensive inflammatory gingiva.
Figures and Tables
Figure 1
CRP Western analysis showing 4 representative samples in each group. CRP levels were quantified on the basis of β-actin levels. CRP corresponding to molecular weight 27 kDa was shown to be expressed in all samples including healthy gingiva, and the expression levels of CRP were increased in order of group 1, group 2 and group 3.
Group 1 : healthy gingiva from systemically healthy person, Group 2 : inflamed gingiva from patient with chronic periodontitis, Group 3 : inflamed gingiva from patient with chronic periodontitis and hypertension.
Figure 2
Graphics showing the average amounts (ratio of CRP/β-actin) and standard deviation of CRP level in groups 1, 2 and 3. In the inflamed gingiva (group 2 and 3), the levels of CRP were significantly increased as compared to group 1 (P<0.05). Group 1 : healthy gingiva from systemically healthy person. Group 2 : inflamed gingiva from patient with chronic periodontitis. Group 3 : inflamed gingiva from patient with chronic periodontitis and hypertension.
+significant difference between group 1 and group 2 (P<0.05). *significant difference between group 1 and group 3 (P<0.05).
Figure 3
M-CSF Western analysis showing 4 representative samples in each group. M-CSF levels were quantified on the basis of β-actin levels. M-CSF corresponding to molecular weight 18.5 kDa was shown to be expressed in all samples including healthy gingiva, and the expression levels of M-CSF were increased in order of group 1, group 2 and group 3. Group 1 : healthy gingiva from systemically healthy person. Group 2 : inflamed gingiva from patient with chronic periodontitis. Group 3 : inflamed gingiva from patient with chronic periodontitis and hypertension.
Figure 4
Graphics showing the average amounts (ratio of M-CSF/β-actin) and standard deviation of M-CSF level in groups 1, 2 and 3. In the inflamed gingiva (group 2 and 3), the levels of M-CSF were significantly increased as compared to group 1 (P<0.05). Group 1 : healthy gingiva from systemically healthy person. Group 2 : inflamed gingiva from patient with chronic periodontitis. Group 3 : inflamed gingiva from patient with chronic periodontitis and hypertension.
+significant difference between group 1 and group 2 (P<0.05). *significant difference between group 1 and group 3 (P<0.05).
Table 2
Normalized CRP expressions by CRP/β-actin
Group 1 : healthy gingiva from systemically healthy person
Group 2 : inflamed gingiva from patient with chronic periodontitis
Group 3 : inflamed gingiva from patient with chronic periodontitis and hypertension
+significant difference between group 1 and group 2 (P<0.05), *significant difference between group 1 and group 3 (P<0.05)
Table 3
Normalized M-CSF expressions by M-CSF/β-actin
Group 1 : healthy gingiva from systemically healthy person
Group 2 : inflamed gingiva from patient with chronic periodontitis
Group 3 : inflamed gingiva from patient with chronic periodontitis and hypertesion
+significant difference between group 1 and group 2 (P<0.05), *significant difference between group 1 and group 3 (P<0.05)
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