Clinical and microbiologic effects of the subantimicrobial dose of doxycycline on the chronic periodontitis

Sang-Jun Kim; Heung-Sik Um; Beom-Seok Chang; Jae-Kwan Lee

doi:10.5051/jkape.2009.39.1.37

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Kim, Um, Chang, and Lee: Clinical and microbiologic effects of the subantimicrobial dose of doxycycline on the chronic periodontitis

Original Article

The Journal of the Korean Academy of Periodontology

The Journal of the Korean Academy of Periodontology 2009; 39(1): 37-44.

Published online: 31 March 2009

DOI: https://doi.org/10.5051/jkape.2009.39.1.37

Clinical and microbiologic effects of the subantimicrobial dose of doxycycline on the chronic periodontitis

Sang-Jun Kim, Heung-Sik Um, Beom-Seok Chang, Jae-Kwan Lee

Department of Periodontology, School of Dentistry, Kangnung National University, Gangneung, Korea.

Correspondence: Dr. Jae-Kwan Lee. Department of Periodontology, School of Dentistry, Kangnung National University, 120 Gangneung Daehangno, Gangneung City, Gangwon Province, 210-702, Korea. periojk@kangnung.ac.kr, Tel: 033-640-3199, Fax: 033-640-3113

Received 11 December 2008 Accepted 7 January 2009

Abstract

Purpose

Tetracycline and its chemically modified non-antibacterial analogues can inhibit certain host-derived tissue destructive collagenases such as matrix metalloproteinases. The purpose of this study was to evaluate clinical and microbiologic effects of the subantimicrobial dose of doxycycline(SDD) in conjunction with scaling and root planing.

Materials and methods

A total of 30 patients with chronic periodontitis who were going to receive scaling and root planing were randomly allocated to receive either a doxycycline hyclate for 3 months or nothing. Clinical probing depth, clinical attachment level, gingival recession, and bleeding on probing were measured by one periodontist. After a periodontal examination, microbial samples were collected using sterile paper points. The effect of SDD in conjunction with scaling and root planing on alterations of the periodontal pathogens (Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis) were also assessed using 16S rRNA polymerase chain reaction.

Results

During the treatment period, clinical parameters for both treatment group and control group were improved. After 3 months, reductions in probing depth and gains in clinical attachment level were significantly greater for the SDD group than control group. Microbial analysis showed that there was no alteration of the periodontal pathogens and no difference between the groups.

Conclusion

This study suggested that the subantimicrobial dose of doxycycline as an adjunct therapy with scaling and root planing might be effective and safe in the management of chronic periodontitis.

Keywords: subantimicrobial dose of doxycycline, chronic periodontitis, periodontal pathogen

Figures and Tables

Figure 1

Study design.

^*SDD: subantimicrobial dose of doxycycline, SRP: scaling and root planing.

Figure 2

Per-patient change in probing depth.

Figure 3

Per-patient change in clinical attachment level.

Figure 4

Electrophoresis results of A. actinomycetemcomitans PCR amplification.

Figure 5

Electrophoresis results of T. forsythia PCR amplification.

Figure 6

Electrophoresis results of P. gingivalis PCR amplification.

Table 1

Species-Specific Primers Used for PCR

Table 2

PCR Thermal Cycle

Table 3

Mean Pocket Depth(Mean±SD; mm)

^*: Statistically significant difference compared to baseline (p<0.01)

^†: Statistically significant difference compared to control group (p<0.05)

^*SDD: subantimicrobial dose of doxycycline

Table 4

Mean Clinical Attachment Level(Mean±SD; mm)

^*: Statistically significant difference compared to baseline (p<0.01)

^†: Statistically significant difference compared to control group (p<0.05)

Table 5

Mean Gingival Recession(Mean±SD; mm)

^*: Statistically significant difference compared to baseline (p<0.05)

Table 6

Bleeding on Probing(Mean±SD; %)

^*: Statistically significant difference compared to baseline (p<0.01)

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ORCID iDs: Jae-Kwan Lee
https://orcid.org/http://orcid.org/0000-0003-1710-1580
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