Sool-Heon Lee; Ji-Il Park; Young-Joon Kim

doi:10.5051/jkape.2009.39.3.359

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Lee, Park, and Kim: Response of fetal rat calvarial cells on mineral trioxide aggregate after IL-1β stimulation

Original Article

The Journal of the Korean Academy of Periodontology

The Journal of the Korean Academy of Periodontology 2009; 39(3): 359-365.

Published online: 30 September 2009

DOI: https://doi.org/10.5051/jkape.2009.39.3.359

Response of fetal rat calvarial cells on mineral trioxide aggregate after IL-1β stimulation

Sool-Heon Lee¹, Ji-Il Park², Young-Joon Kim¹

¹Department of Periodontology, School of Dentistry, Dental Science Reserch Institute, Chonnam National University, Korea.

²Department of Dental hygiene, Gwangju Health College, Korea.

Correspondence: Dr. Young-Joon Kim. Department of Periodontology, School of Dentistry, Dental Science Reserch Institute, Chonnam National University, 333, Yong Bong Ro, Buk-gu, Gwang-Ju, 500-757, Korea. youngjun@chonnam.ac.kr, Tel: 062-530-5648, Fax: 062-530-5649

Received 12 August 2009 Accepted 17 September 2009

Abstract

Purpose

The purpose of this study was to investigate the ability of Mineral trioxide aggregate(MTA) to support osteoclastic differentiation from fetal rat calvarial cell.

Methods

In this study, response of IL-6, RANKL, and OPG in fetal rat calvarial cells stimulated with IL-1β on MTA was evaluated by ELISA and RT-PCR.

Results

The results were as follows; there was no significant difference between glass and MTA at 5days. In ELISA analysis, Glass group and MTA group showed similar IL-6 expression, Glass+IL-1β group and MTA+IL-1β group showed similar IL-6 expression. In RT-PCR analysis, Glass group and MTA group showed similar IL-6, RANKL, OPG mRNA expression, MTA+IL-1β group and Glass+IL-1β group showed 3 fold increase of IL-6 and RNAKL mRNA expression when compared with MTA group. All groups showed similar OPG mRNA expression.

Conclusions

MTA does not suppress cell proliferation and increase the proinflammatory cytokine that induce osteoclastogenesis. Thus, MTA is biocompatible material that could be used in various clinical conditions.

Keywords: biocompatible materials, cytokines, ELISA, mineral trioxide aggregate

Figures and Tables

Figure 1

Cell proliferation assay after 3 days and 5 days of culture on glass and MTA.

^*: significantly different compared to glass at day 3 (p<0.05).

Figure 2

Mean concentration of IL-6 in glass, MTA, MTA+IL-1β, glass+IL-1β.

^*: Significantly different to Glass at day 1 (p<0.05).

^†: Significantly different to MTA only at day 1 (p<0.05).

^§: Significantly different to Glass at day 3 (p<0.05).

Figure 3

Reverse transcription-polymerase chain reaction (RTc-PCR) analysis of IL-6, RANKL and OPG mRNA expression related to GAPDH means in rat calvarial cells.

Table 1

Amplication Primer Sets Used in Polymerase Chain Reaction

GAPDH indicate glyceraldehyde-3-phosphate dehydrohenase; RANKL, receptor activation of nuclear factor κB ligand; OPG, osteoprotegerin; IL-6, interleukin-6.

Table 2

Cell Proliferation Assay After 3 Days and 5 Days of Culture on Glass and MTA

^*: Significantly different compared to glass at day 3 (p < 0.05).

Table 3

Mean Concentration of IL-6 in Cell Culture on Glass, MTA, MTA+IL-1β, Glass+IL-1β (pg/ml)

^*: Significantly different to Glass at day 1 (p < 0.05).

^†: Significantly different to MTA at day 1 (p < 0.05).

^§: Significantly different to Glass at day 3 (p < 0.05).

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