The effects of bone regeneration in rabbit calvarial defect with particulated and block type of hydroxyapatite

Sun-Ja Gu; Joo-Yeon Sohn; Hyun-Chang Lim; Yoo-Jung Um; Ui-Won Jung; Chang-Sung Kim; Yong-Keun Lee; Seong-Ho Choi

doi:10.5051/jkape.2009.39.3.321

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Gu, Sohn, Lim, Um, Jung, Kim, Lee, and Choi: The effects of bone regeneration in rabbit calvarial defect with particulated and block type of hydroxyapatite

Original Article

The Journal of the Korean Academy of Periodontology

The Journal of the Korean Academy of Periodontology 2009; 39(3): 321-329.

Published online: 30 September 2009

DOI: https://doi.org/10.5051/jkape.2009.39.3.321

The effects of bone regeneration in rabbit calvarial defect with particulated and block type of hydroxyapatite

Sun-Ja Gu¹, Joo-Yeon Sohn¹, Hyun-Chang Lim¹, Yoo-Jung Um¹, Ui-Won Jung¹, Chang-Sung Kim¹, Yong-Keun Lee², Seong-Ho Choi¹

¹Department of Periodontology, Research institute of periodontal regeneration, College of Dentistry, Yonsei University, Korea.

²Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Korea.

Correspondence: Dr. Seong-Ho Choi. Department of Periodontology, College of Dentistry, Yonsei University, 134 Shinchon-dong Seodaemun-gu, Seoul, 120-752, Korea. shchoi726@yuhs.ac, Tel: 02-2228-3189, Fax: 02-392-0398

Received 8 June 2009 Accepted 9 August 2009

Abstract

Purpose

The purpose of this study was to evaluate the bone regeneration of particulated hydroxyapatite(HA) and block type of hydroxyapatite graft in rabbit calvarial defects.

Methods

An 8 mm calvarial circular defects were created in sixteen young adult New Zealand white male rabbits (weight 3.0~3.5 kg). Each defects were filled with Bio-Oss, particulated HA and block type HA. Sham surgery control defects were filled with blood clots. The specimens were harvested at 4 weeks and 8 weeks for histologic and histomorphometric evaluation.

Results

Histomorphometric analysis demonstrated statistical differences in defect closure, new bone formation, and bone density of the four groups. Block type of HA group showed increased bone formation and bone density at 4 weeks and 8 weeks compared with Bio-Oss group or sham surgery control group(p<0.05).

Conclusions

Block type of HA is an effective material for osteoconduction in rabbit calvarial defects, which may acts as a guide in use of these products in human application.

Keywords: bone regeneration, bone substitutes, hydroxyapatites

Figures and Tables

Figure 1

Calvarial defect formation.

Figure 2

Calvarial defects were filled with block type HA, particulated HA, Bio-Oss.

Figure 3

Histologic section of control group at 4 weeks (×10, H-E). Arrow head: defect margin.

Figure 4

Histologic section of control group at 8 weeks (×10, H-E). Arrow head: defect margin.

Figure 5

Histologic section of Bio-Oss group 4 weeks (×10, H-E). Arrow head: defect margin.

Figure 6

Histologic section of Bio-Oss group at 8 weeks (×10, H-E). Arrow head: defect margin.

Figure 7

Higher magnification of Bio-Oss group presenting new bone (NB) with Bio-Oss remnants (arrow). (×100, H-E).

Figure 8

Histologic section of particulated HA group at 4 weeks (×10, H-E). Arrow head: defect margin.

Figure 9

Histologic section of particulated HA group at 8 weeks (×10, H-E). Arrow head: defect margin.

Figure 10

Higher magnification of particulated HA group presenting new bone (NB) with HA remnants (arrow). (×100, H-E).

Figure 11

Histologic section of block type HA group at 4 weeks (×10, H-E). Arrow head: defect margin.

Figure 12

Histologic section of block type HA group at 8 weeks (×10, H-E). Arrow head: defect margin.

Figure 13

Higher magnification of block type HA group presenting new bone (NB) with osteoblastic steam (arrow). (×100, H-E).

Figure 14

Higher magnification of block type HA group showing new bone with numerous blood vessel (×100, H-E).

Table 1

Histomorphometric Analysis of Defect closure (%) (Mean±Standard Deviation; n=8)

^*Statistically significant difference compared to sham surgery control group (p<0.05)

^†Statistically significant difference compared to Bio-Oss group (p<0.05)

Table 2

Histomorphometric Analysis of Augmented area (mm²) (Mean±Standard Deviation; n=8)

^*Statistically significant difference compared to sham surgery control group (p<0.05)

^†Statistically significant difference compared to Bio-Oss group (p<0.05)

Table 3

Histomorphometric Analysis of New bone area (mm²) (Mean±Standard Deviation; n=8)

^*Statistically significant difference compared to sham surgery control group (p<0.05)

^†Statistically significant difference compared to Bio-Oss group (p<0.05)

Table 4

Histomorphometric Analysis of Bone density (%) (Mean±Standard Deviation; n=8)

^*Statistically significant difference compared to sham surgery control group (p<0.05)

^†Statistically significant difference compared to Bio-Oss group (p<0.05)

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