Journal List > J Korean Acad Periodontol > v.38(Suppl) > 1049765

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Lee, Kim, Seol, Lee, Ku, Rhyu, Chung, and Han: Effects of enamel matrix derivatives on biologic activities of human periodontal fibloblasts to demineralized root surface
Original Article

The Journal of the Korean Academy of Periodontology 2008; 38(4): 679-690.

Published online: 31 December 2008

DOI: https://doi.org/10.5051/jkape.2008.38.4.679

Effects of enamel matrix derivatives on biologic activities of human periodontal fibloblasts to demineralized root surface

Kang-Woon Lee, Tae-Il Kim, Yang-Jo Seol, Yong-Moo Lee, Young Ku, In-Chul Rhyu, Chong-Pyung Chung, Soo-Boo Han

Department of Periodontology, School of dentistry, Seoul National University, Korea.

Correspondence: Dr. Soo-Boo Han. Department of Periodontology, College of dentistry, Seoul National University, 28 Yeongon-Dong, Chongno-Ku, Seoul, 110-744, Korea. perioh@snu.ac.kr, Tel: 82-2-2072-2644, Fax: 82-2-744-0051

Received 3 November 2008       Accepted 18 December 2008

Copyright © 2008 Korean Academy of Periodontology

Abstract

Purpose

The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA).

Material and Methods

Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and 100 microgram/ml concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done.

Results

Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days.

Conclusion

Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD.

Keywords: Enamel matrix derivatives, PDL cell, Tetracycline, EDTA

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