Journal List > J Korean Acad Periodontol > v.38(Suppl) > 1049761

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Choi, Jang, and Kim: Effects of H2O2 and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts
Original Article

The Journal of the Korean Academy of Periodontology 2008; 38(4): 645-656.

Published online: 31 December 2008

DOI: https://doi.org/10.5051/jkape.2008.38.4.645

Effects of H2O2 and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts

Seong-Mi Choi1, Hyun-Seon Jang1, 2, Byung-Ock Kim1, 2

1Department of Periodontology, School of Dentistry, Cho Sun University, Korea.

2The 2nd stage of BK21, Korea.

Correspondence: Dr. Byung-Ock Kim. Department of Periodontology, School of Dentistry, Chosun University, Seosuk-Dong, Dong-gu, Gwang-Ju, 501-759, Korea. bobkim@chosun.ac.kr, Tel: 82-62-220-3854, Fax: 82-62-224-4664

Received 29 October 2008       Accepted 10 December 2008

Copyright © 2008 Korean Academy of Periodontology

Abstract

Purpose

To evaulate the effects of chlorhexidine and H2O2 on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF).

Materials and Methods

1.2×10-1%, 1.2×10-2% and 1.2×10-3% CHX and 3×10-3%, 3×10-4% and 3×10-5% H2O2 and mixture of CHX and H2O2 were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR.

Results

The result were as follows:

  1. The expression of UNCL mRNA was higher than that of other mRNAs.

  2. 1.2×10-3 % CHX increased mRNA expressions of hPDLF as application time increased.

  3. H2O2 lower than 3×10-3 % increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF.

  4. hPDLF treatment with 1.2×10-1 % CHX (with or without H2O2) resulted in no gene expression.

  5. hPDLF treatment with 1.2×10-2 % CHX (with or without H2O2) for 30 minutes resulted in no gene expression.

Conclusion

Because low concentration of CHX and H2O2 increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect.

Keywords: chlorhexidine, H2O2, human periodontal ligament fibroblasts

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