Journal List > J Korean Acad Periodontol > v.38(Suppl) > 1049748

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Kim, Lee, Choi, Koo, Kim, Lee, Ku, Chung, and Rhyu: Pro-inflammatory cytokine expression in human gingival fibroblasts by Tannerella forsythia whole bacteria, membrane proteins, and lipopolysaccharide
Original Article

The Journal of the Korean Academy of Periodontology 2008; 38(3): 543-550.

Published online: 30 September 2008

DOI: https://doi.org/10.5051/jkape.2008.38.3.543

Pro-inflammatory cytokine expression in human gingival fibroblasts by Tannerella forsythia whole bacteria, membrane proteins, and lipopolysaccharide

Jung-Eun Kim1, Sung-Hoon Lee2, Bong-Kyu Choi2, Ki-Tae Koo1, Tae-Il Kim1, Yong-Moo Lee1, Young Ku1, Chong-Pyoung Chung1, In-Chul Rhyu1

1Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Korea.

2Oral Infection and Immunity, School of Dentistry, Seoul National University, Korea.

Correspondence: In-Chul Rhyu. Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, 28 Yeongon-dong, Chongno-ku, Seoul, 110-744, Korea. icrhyu@snu.ac.kr, Tel: 82-2-2072-2640, Fax: 82-2-744-0051

Received 5 September 2008       Accepted 12 September 2008

Copyright © 2008 Korean Academy of Periodontology

Abstract

Purpose

The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia.

Material and Methods

After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia, the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria.

Result

The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-1beta was induced by membrane protein of T. forsythia, not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs.

Conclusion

The membrane protein of T. forsythia could be one of virulence factors.

Keywords: Tannerella forsythia, virulence factors, human gingival fibroblasts

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