Journal List > J Korean Acad Periodontol > v.38(Suppl) > 1049726

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Kim, Yun, Chae, Jung, Kim, and Cho: rhBMP-2 using biphasic calcium phosphate block as a carrier induces new bone formation in a rat subcutaneous tissue
Original Article

The Journal of the Korean Academy of Periodontology 2008; 38(Suppl): 355-362.

Published online: 31 August 2008

DOI: https://doi.org/10.5051/jkape.2008.38.Suppl.355

rhBMP-2 using biphasic calcium phosphate block as a carrier induces new bone formation in a rat subcutaneous tissue

Joon-Il Kim1, Jeong-Ho Yun2, Gyung-Joon Chae1, Sung-Won Jung1, Chang-Sung Kim1, Kyoo-Sung Cho1

1Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Korea.

2Department of Dentistry, College of Medicine, Kwandong University, Myongji Hospital, Korea.

Correspondence: Dr. Kyoo-Sung Cho. Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, 134 Shinchon-Dong, Seodaemun-gu, Seoul, 120-752, Korea. kscho@yuhs.ac, Tel: 82-2-2228-3188, Fax: 82-2-392-0398

Received 16 May 2008       Accepted 4 July 2008

Copyright © 2008 Korean Academy of Periodontology

Abstract

Purpose

The carrier for the delivery of bone morphogenetic proteins(BMPs) should also serve as a scaffold for new bone growth. In addition, predictable bone formation in terms of the volume and shape should be guaranteed. This study evaluated the ectopic bone formation of recombinant human BMP-2(rhBMP-2) using a micro macroporous biphasic calcium phosphate (MBCP: mixture of βTCP and HA) block as a carrier in a rat subcutaneous assay model.

Materials and Methods

Subcutaneous pockets were created on the back of 40 male Sprague-Dawley rats. In the pockets, rhBMP-2/MBCP and MBCP alone were implanted. The blocks were evaluated by histological and histometric parameters after a healing interval of 2 weeks (each 10 rats; MBCP and rhBMP-2/MBCP) or 8 weeks (each 10 rats; MBCP and rhBMP-2/MBCP).

Results

The shape and volume of the block was maintained stable over the healing period. No histological bone forming activity was observed in the MBCP alone sites after 2 weeks and there was minimal new bone formation at 8 weeks. In the rhBMP-2/MBCP sites, new bone formation was evident in the macropores of the block. The new bone area at 8 weeks was greater than at 2 weeks. There was a further increase in the quantity of new bone with the more advanced stage of remodeling.

Conclusions

A MBCP block could serve as a carrier system for predictable bone tissue engineering using rhBMPs.

Keywords: rhBMP-2, biphasic calcium phosphate block, carrier, ectopic bone formation

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