Journal List > J Korean Acad Periodontol > v.37(Suppl) > 1049660

TOOLS
Jung, Kim, Yook, Kim, Kim, and Cho: Bone regenerative effects of recombinant human bone morphogenetic protein-2 employed protein transduction domain
Original Article

The Journal of the Korean Academy of Periodontology 2007; 37(3): 497-509.

Published online: 30 September 2007

DOI: https://doi.org/10.5051/jkape.2007.37.3.497

Bone regenerative effects of recombinant human bone morphogenetic protein-2 employed protein transduction domain

Sung-Won Jung1, Nam-Hee Kim2, Jong-In Yook2, Chang-Sung Kim1, Hyung-Jun Kim3, Kyoo-Sung Cho1

1Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University, Korea.

2Department of Oral pathology, Oral Cancer Research institute, College of Dentistry, Yonsei University, Korea.

3Department of Oral & Maxillofacial surgery, College of Dentistry, Yonsei University, Korea.

Corresponding Author (kscho@yumc.yonsei.ac.kr)

Copyright © 2007 Korean Academy of Periodontology

Abstract

Bone morphogenetic proteins(BMPs) are regarded as members of the transforming growth factor-β superfamily with characteristic features in their amino acid sequences. A number of studies have demonstrated the biologic activities of BMPs, which include the induction of cartilage and bone formation. Recently there was a attempt to overcome a limitation of mass production, and economical efficieny of rh-BMPs. The method producing PTD by using bacteria have advantages of acquiry a mass of proteins. Hences, a new treatment which deliver protein employed by protein transduction domain(PTD) has been tried.
The purpose of this study was to evaluate the bone regenerative effect of TATBMP-2 and TAT-HA2-BMP-2 employed by PTD from HIV-1 TAT protein for protein translocation in the rat calvarial model. An 8mm calvarial, critical size osteotomy defect was created in each of 32 male Spraque-Dawley rats(weight 250~300g). The animals were divided into 4 groups of 32 animals each (4 animals/group/healing interval). The defect was treated with TATBMP-2/ACS(Absorbable collagen sponge) (TATBMP-2 0.1mg/ml), TAT-HA2-BMP-2/ACS(TAT-HA2-BMP-2 0.1mg/ml), ACS alone or left untreated for surgical control(negative control). The rats were sacrificed at 2 or 8 weeks postsurgery, and the results were evaluated histologically. The results were as follows:
New bone formation were not significantly greater in the TATBMP-2/ACS group relative to negative, and positive control groups.
New bone was evident at the defect sites in TAT-HA2-BMP-2/ACS group relative to negative, positive control and TATBMP-2 groups.
There were a little bone regeneration in TATBMP-2 groups. While, enhanced local bone formation were observed in TAT-HA2-BMP-2 group. But, The results was not the same in all rat defects. Therefore, further investigations are required to develop a method, which disperse homogenously, and adhere to target cells.

Keywords: bone regeneration, Bone morphogenetic protein, protein transduction domain

TOOLS
Similar articles