Journal List > J Korean Acad Periodontol > v.36(3) > 1049608

J Korean Acad Periodontol. 2006 Sep;36(3):757-765. Korean.
Published online September 30, 2006.  https://doi.org/10.5051/jkape.2006.36.3.757
Copyright © 2006 Korean Academy of Periodontology
Periodontal tissue engineering by hPDLF seeding on scaffold
Seong Sin Kim,1 Byung-Ock Kim,1,3 Joo-Cheol Park,2,3 and Hyun Seon Jang1,3
1Department of Periodontology, College of Dentistry, Chosun University, Korea.
2Department of Oral Histology, College of Dentistry, Chosun University, Korea.
3Oral Biology Research Institute, College of Dentistry, Chosun University, Korea.

Corresponding author (Email: periojang@chosun.ac.kr )
Abstract

Human periodontal ligament fibroblasts (hPDLF) are very important for curing the periodontal tissue because they can be differentiated into various cells. A tissue engineering approach using a cell-scaffold is essential for comprehending today's periodontal tissue regeneration procedure. This study examined the possibility of using an acellular dermal matrix as a scaffold for human periodontal ligament fibroblast (hPDLF). The hPDLF was isolated from the middle third of the root of periodontally healthy teeth extracted for orthodontic reasons. The cells were cultured in a medium containing Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum at 37℃ in humidified air with 5% CO2. The acellular dermal matrix(ADM) was provided by the US tissue banks(USA). Second passage cells were used in this study. The hPDLF cells were cultured with the acellular dermal matrix for 2 days, and the dermal matrix cultured by the hPDLF was transferred to a new petri dish and used as the experimental group. The control group was cultured without the acellular dermal matrix. The control and experimental cells were cultured for six weeks. The hPDLF cultured on the acellular dermal matrix was observed by Transmission Electron microscopy (TEM). Electron micrography shows that the hPDLF was proliferated on the acellular dermal matrix. This study suggests that the acellular dermal matrix can be used as a scaffold for hPDLF.

Keywords: human periodontal ligament fibroblast(hPDLF); acellular dermal matrix; transluscent electronmicroscope(TEM); scaffold