The purpose of this study was to investigate the effects of irradiated frozen allogenic bone(IFAB) on the cell proliferation and differentiation of human fetal osteoblasts. Human fetal osteoblasts(hFOB1) were cultured to examine the cellular proliferation for 3 days and 5 days with 1mg/ml, 100µg/ml, 10µg/ml, 1µg/ml, 100ng/ml, 10ng/ml, 1ng/ml of IFAB, and to compare the ALP synthesis to control groups for 3 days with DMEM/F-12 1:1 Mixture and 1mg/ml, 100µg/ml, 10µg/ml, 1µg/ml, 100ng/ml, 10ng/ml, 1ng/ml of IFAB. To compare the calcium accumulation, hFOB1 cultured for 23 days were quantified and photographed. The cellular proliferation of hFOB1s treated with IFAB was increased at 5 days to control(p<0.05). The activity of ALP in hFOB1s treated with 100ng/ml IFAB was significantly increased at 5 days(p<0.05). A quantified calcium accumulation in hFOB1 was significantly increased at 100ng/ml, 10ng/ml of IFAB(p<0.05).