Journal List > J Korean Acad Periodontol > v.35(3) > 1049523

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Nam, Moon, Lee, Cha, Choi, and Yoo: Effect of Treponema lecithinolyticum lipopolysaccharide on matrix metalloproteinase-9 expression
Original Article

The Journal of the Korean Academy of Periodontology 2005; 35(3): 675-685.

Published online: 30 September 2005

DOI: https://doi.org/10.5051/jkape.2005.35.3.675

Effect of Treponema lecithinolyticum lipopolysaccharide on matrix metalloproteinase-9 expression

Jeong Ah Nam1, Sun Young Moon1, Jin Wook Lee1, Jeong Heon Cha1, Bong Kyu Choi1, Yun Jung Yoo1

Department of Oral Biology, College of Dentistry, Yonsei University, Seoul, Korea.

Department of Oromaxillofacial Infection and Immunity, and Dental Research Institute, College of Dentistry, Seoul National University, Seoul, Korea.

Corresponding author (yu618@yumc.yonsei.ac.kr)

Copyright © 2005 Korean Academy of Periodontology

Abstract

Bone resorption involves sequential stages of osteoclast precursor migration and differen-tiation of osteoclast precursors into multinucleated osteoclasts. Stromal cell derived factor (SDF)-1 is a chemotactic factor for osteoclast precursor migration. Matrix metalloproteinase (MMP)-9 is involved in migration of osteoclast precursors and activation of interleukin(IL)-1β. Alveolar bone destruction is a characteristic feature of periodontal disease. Treponema lecithinolyticum is a oral spirochete isolated from the periodontal lesions. The effect of lipopolysaccharide(LPS) from T. lecithinolyticum on expression of SDF-1 and MMP-9 was examined in cocultures of bone marrow cells and osteblasts derived from mouse calvariae.
T. lecithinolyticum LPS increased expression of MMP-9 in the coculture. Polymyxin B, an inhibitor of LPS, abolished the increase of MMP-9 mRNA expression by LPS. LPS did not increase the expression of SDF-1, IL-1β and tumor necrosis factor(TNF)-α mRNA in cocultures. Prostaglandin E2(PGE2) up-regulated the expression of MMP-9 and NS398, an inhibitor of PGE2 synthesis, down-regulated the induction of MMP-9 expression by T. lecithinolyticm LPS.
These results suggest that T. lecithinolytium LPS increases MMP-9 expression in bone cells via PGE2 and that the induction of MMP-9 expression by T. lecithinolyticum LPS is involved in alveolar bone destruction of periodontitis patients by the increase of osteoclast precursor migration and the activation of bone resorption-inducing cytokine.

Keywords: Treponema lecithinolyticum, lipopolysaccharide, MMP-9

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