After titanium implant were installed in the proximal tibial metaphysis of New Zealand white rabbit, negatively charged PTFE membrane fabricated by corana dischage was inserted into the inner hole of the experimental implant and noncharged membrane was applied into control implant. After 4 weeks of healing, histomorphometric analysis was performed to evaluate peri-implant bone response. The histomorphometric evaluations demonstrated experimental implant tended to have higher values in the total bone-to-implant contact ratio(experimental ; 49.9±13.52% vs control ; 37.5±19.44%) , the marrow bone contact ratio(experimental ; 34.94±13.32% vs control ; 24.15±13.69%), amount of newly formed bone in the endosteal region(experimental ; 1.00±0.30mm vs control ; 0.61±0.24mm) and bone area in the medullary canal(experimental ; 13.55±4.98% vs control ; 9.03±3.05%). The mean values of the amount of newly formed bone(endosteal region) and bone area(medullary canal) of the experimental implant demonstrated a statistically significant difference as compared to the control implant(p<0.05).

In conclusion, noninvasive electrical stimulation using negatively charged electret effectively promoted peri-implant new bone formation in this study. This method is expected to be used as one of the useful electrical stimulation for enhancing bone healing response in the implant therapy