Journal List > J Korean Acad Periodontol > v.34(4) > 1049460

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Kim, Yun, Jung, Yoo, Kim, You, Kim, and Choi: The Effects of Dichloromethane fraction of Phlomodis Radix(DFPR) on differentiation of Mouse Calvarial Cell
Original Article

The Journal of the Korean Academy of Periodontology 2004; 34(4): 791-805.

Published online: 31 December 2004

DOI: https://doi.org/10.5051/jkape.2004.34.4.791

The Effects of Dichloromethane fraction of Phlomodis Radix(DFPR) on differentiation of Mouse Calvarial Cell

Dong-Jin Kim1, Jeong-Ho Yun1, Ui-Won Jung1, Yun-Jung Yoo2, Yun-Chul Kim3, Hyung-Keun You4, Chong-Kwan Kim5, Sung-Ho Choi5

1Department of Periodontology, College of Dentistry, Yonsei University, Korea.

2Department of Oral Biology, College of Dentistry, Yonsei University, Korea.

3Department of Pharmacy, Pharmacy College, Wonkwang University, Korea.

4Department of Periodontology, School of Dentistry, Wonkwang University, Korea.

5Department of Periodontology, College of Dentistry, Yonsei University, Brain Korea 21 Project for Medical Science, Korea.

Copyright © 2004 Korean Academy of Periodontology

Abstract

The purpose of this study was to evaluate the effects of DFPR on differentiation of mouse calvarial cell in vitro, to examine the possibility for periodontal regeneration. 10µg/ml of DFPR was used as experimental concentration. osteogenic medium only was assigned as control, Experimental 1 was supplemented with 10nM dexamethasone, Experimental 2 with 10µg/ml DFPR and Experimental 3 with 10nM dexamethasone + 10microgram/ml DFPR. cellular activity was evaluated by MTT method at 8, 12, 16 days, expression of mRNA of ALP, osteopontin, osteocalcin, collagen type-1 was detected by RT-PCR method at 4, 8, 12, 16 days of culture. extent of mineralization was observed by Von Kossa staining at 16 day of culture.
The results are as follows 1)Any acceleration of differentiation was not observed at expression of differentiation marker, 2) Decrease in expression of extracelluar matrix and in bone nodule formation was observed.
The results suggested that DFPR have negative effect on the rate of differentiation on rat calvarial cell, decrease extracelluar matrix formation ,decrease bone nodule formation. Ongoing studies are necessary in order to determine effect of DFPR on periodontal regeneration.

Keywords: DFPR, mouse calvarial cell, differentiation

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