Journal List > J Korean Acad Periodontol > v.34(4) > 1049458

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Han, Ciu, Kim, Chung, and Kim: Effects of the MnCl2 on bone formation in fetal rat calvarial cell
Original Article

The Journal of the Korean Academy of Periodontology 2004; 34(4): 771-780.

Published online: 31 December 2004

DOI: https://doi.org/10.5051/jkape.2004.34.4.771

Effects of the MnCl2 on bone formation in fetal rat calvarial cell

Woong-Taek Han1, De-Zhe Ciu2, Young-Joon Kim1, 3, Hyun-Ju Chung1, 3, Ok-Su Kim1, 3

1Department of Periodontology, Chonnam National University, Korea.

2Graduate School Department of Dental Science, Chonnam National University, Korea.

3Dental Science Research Institute, Chonnam National University, Korea.

Corresponding author (youngjun@jnu.ac.kr)

Copyright © 2004 Korean Academy of Periodontology

Abstract

Chronic exposure to high levels of manganese (Mn) leads a pronounced and debilitating disorder known as manganism. Research on the toxic manifestation of manganese have focused primarily on its neurological effects because exposure to high levels of the metal produces a distinct and irreversible extrapyramidal dysfunction resembling the dystonic movements associated with Parkinson's physiological and biochemical systems in the body.
The purpose of this study is to determine the effects of Mn on mineralization in primary rat calvarial cells. The experimental groups were in concentration of 0, 10, 30 and 60 µm.
The results were as follows:
1. ALP activity was decreased in concentration of 30 and 60 µm (p<0.01).
2. Bone nodule formation was depressed in concentration of 30 and 60 µm at day 14 and 21 (p<0.01).
3. RT-PCR results showed an altered expression of bone matrix proteins.
These result suggested that manganese might decrease or alter the expression of the osteoblast phenotype.

Keywords: MnCl2, rat calvarial cell, ALP, osteoblast phenotype

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