Journal List > J Korean Acad Periodontol > v.34(1) > 1049432

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Kim, Kim, Lee, Lee, Cho, Chai, Kim, and Choi: The Effects of Calcium Phosphate Glass on Mouse Calvarial Cell
Original Article

The Journal of the Korean Academy of Periodontology 2004; 34(1): 49-59.

Published online: 31 March 2004

DOI: https://doi.org/10.5051/jkape.2004.34.1.49

The Effects of Calcium Phosphate Glass on Mouse Calvarial Cell

Min-Kyoung Kim1, Chang-Sung Kim1, Doug-Youn Lee2, 3, Yong-Keun Lee3, Kyoo-Sung Cho1, 2, Jung-Kiu Chai1, Chong-Kwan Kim1, 2, Seong-Ho Choi1, 2

1Department of Periodontology, College of Dentistry, Yonsei University, Research Institute for Periodontal Regeneration, Korea.

2Brain Korea 21 Project for Medical Science, Korea.

3Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Korea.

Copyright © 2004 Korean Academy of Periodontology

Abstract

The goal of periodontal treatment is not only to arrest the progression of the disease but also to promote the functional, esthetic regeneration of the periodontium. Flap operation, bone graft, guided tissue regeneration, growth factors and bone morphogenetic protein have been used for this purpose. Among these techniques of regeneration, alloplastic graft, especially calcium phosphate is getting more attention recently.
The purpose of this study was to evaluate the effects of calcium phosphate glass on mouse calvarial cell in vitro. The toxicity of calcium phosphate glass was measured using MTT assay, the synthesis of collagen was measured using collagen assay, and ALP activity was measured. The experimental groups were cultured with calcium phosphate glass(both AQ-, and HT-CPG) in concentration of 0.01, 0.02, 0.1, 0.2g/ml.
The results are as follows
1. In concentrations not exceeding 0.02g/ml, both the groups(AQ-CPG, HT-CPG) didn't show any toxicity on mouse calvarial cell(p<0.05).
2. In both the experimental groups are the concentration of 0.02g/ml, collagen expressions were significantly up-regulated (p<0.05).
3. In both the experimental groups are the concentration of 0.02g/ml, ALP activity was not significantly upregulated, but ALP activity in both experimental groups were greater than control group(p<0.05).
The results suggested that the use of calcium phosphate glass may promotes periodontal regeneration. Ongoing studies are necessary in order to determine their regeneration effects.

Keywords: calcium phosphate glass, mouse calvarial cell, MTT assay, collagen assay, ALP activity

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