The purpose of the present study was to investigate the effects of nicotine on bone mineralization in human fetal osteoblasts cell line(hFOB1). To compare the alkaline ph-osphatase(ALP) synthesis, hFOB1 were cultured with DMEM/F-12 1:1 Mixture and 100 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, 1 µ/ml, 10 µ/ml, 100 µ/ml of nicotine. And to compare the calcium accumulation, hFOB1 cultured for 23 days were quantified and photographed.

ALP activity of hFOB1 exposed to nicotine was not significantly changed at a lower concentrations of nicotine, but was significantly decreased at a higher concentrations (10 µ/ml, 100 µ/ml) of nicotine (p<0.05). A quantified calcium acculation in hFOB1 was significantly decreased at 1, 10, and 100µ/ml of nicotine (p<0.05). Significantly decreased calcium deposition was observed at 1, 10, and 100µ/ml of nicotine.

These results indicate that a higher concentration of nicotine show a negative effects on mineralization of hFOB1.