Journal List > J Korean Acad Periodontol > v.33(1) > 1049339

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Nam, Kim, Kim, Yang, Shin, Lee, Chung, Han, Choi, and Rhyu: Biological Activities of HA-coated Zirconia
Original Article

The Journal of the Korean Academy of Periodontology 2003; 33(1): 1-11.

Published online: 31 March 2003

DOI: https://doi.org/10.5051/jkape.2003.33.1.1

Biological Activities of HA-coated Zirconia

Suk-Woo Nam1, Hae-Won Kim2, Hyoun-Ee Kim2, Seung-Min Yang1, 3, Seung-Youn Shin1, Yong-Moo Lee1, Chong-Pyoung Chung1, Soo-Boo Han1, Sang-Mook Choi1, In-Chul Rhyu1

1Department of Periodontology, College of Dentistry, Seoul National University, Korea.

2School of Materials Science and Engineering, Seoul National University, Korea.

3Department of Dentistry, College of Medicine, Sungkyunkwan University, Korea.

Copyright © 2003 Korean Academy of Periodontology

Abstract

Hydroxyapatite(HA) has been extensively used as bone graft materials and tooth implant surface coating materials because of its biocompatibility and osteoconductive properties. However, as HA is intrinsically poor in mechanical properties, zirconia(ZrO2) was incorporated with HA as reinforcing phases for improvement of mechanical properties.
The purpose of this study was to investigate the biological activities of HA-coated zirconia through the cell proliferation test, measurements of alkaline phosphatase activity, and histologic examination. Four kinds of tested blocks were prepared according to the pore size (300-500µM/500-700µM) and the porosity (70%/90%).
Cell proliferation and alkaline phosphatase activity was measured at 1, 7, 14 days. The number of cells proliferate after 7, 14 days were significantly increased in all groups when compared with that of the first day, but there was no significant difference between the 4 groups at each time period. At the 7 day, alkaline phosphatase activities of cells cultured in 4 groups were higher than that of the first day, but there was no significant difference between the 4 groups at each time period.
The human gingival fibroblast and MG 63 cell was used to evaluate the cell cytotoxicity using MTT test. The materials tested in the current study turned out to be non-cytotoxic.
In histologic examination(SEM), at 1 day there were many cells attached on the surfaces of all kinds of tested blocks. The number of cells were increased over time. At the 14 day, there were more cells proliferated than 1 day and some of the pores of blocks were partially filled with the proliferated cells.
The in vitro response of osteoblast-like cells to the HA-coated zirconia showed comparable effect on transformation comparable to hydroxyapatite.

Keywords: Hydroxyapatite, ZrO2, Biological activity, Osteoconduction

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