Journal List > J Korean Acad Periodontol > v.32(2) > 1049285

TOOLS
Gang, Kim, Kim, Seong, Kim, Han, and Kook: Study on isolation of Prevotella nigrescens 9336-specific DNA probes using random cloning method
Original Article

The Journal of the Korean Academy of Periodontology 2002; 32(2): 269-279.

Published online: 30 June 2002

DOI: https://doi.org/10.5051/jkape.2002.32.2.269

Study on isolation of Prevotella nigrescens 9336-specific DNA probes using random cloning method

Soon Won Gang, Se Hoon Kim, Dong Kie Kim, Jin-Hyo Seong, Byung-Ock Kim, Jin-Ju Han, Joong-Ki Kook

1Department of Periodontology, College of Dentistry, Chosun University, Korea.

2Department of preventive Dentistry, College of Dentistry, Chosun University, Korea.

3Department of Oral Biochemistry, College of Dentistry, Chosun University, Korea.

Copyright © 2002 Korean Academy of Periodontology

Abstract

The purpose of this study is to develop species-specific DNA probes and polymerase chain reaction (PCR) primers for detection and identification of Prevotella nigrescens (P. nigrescens) 9336. This study procedure includes (1) whole-genomic DNA extraction of P. nigrescens 9336 (2) construction of the genomic DNA library, (3) screening of strain-specific DNA probe by reverse dot Hybridization method, (4) confirmation of strain-specific DNA probe by Southern blot analysis, (5) determination of nucleotide sequences of strain-specific DNA probe. Thirty-five restriction fragments of P. nigrescens 9336 genomic DNA digested with the Hind III were obtained. Reverse dot hybridization and Southern blot analysis data showed that three of them, Pn10, Pn23, and Pn35, could be P. nigrescens 9336-specific DNA probes. These data indicated that these DNA probes could be useful in detection and identification of the P. nigrescens 9336.

Keywords: Prevotella nigrescens 9336, DNA probe

TOOLS
Similar articles