Journal List > J Korean Acad Periodontol > v.30(2) > 1049161

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Yang, Lee, and Suh: The Effect of 17β-Estradiol on the Gene Expression of IGF-I and Bone Matrix Protein in the Osteoblast-Like Cell
Original Article

The Journal of the Korean Academy of Periodontology 2000; 30(2): 375-388.

Published online: 30 June 2000

DOI: https://doi.org/10.5051/jkape.2000.30.2.375

The Effect of 17β-Estradiol on the Gene Expression of IGF-I and Bone Matrix Protein in the Osteoblast-Like Cell

Won-Suk Yang, Jae-Mok Lee, Jo-Young Suh

Department of Periodontology, School of Dentistry, Kyungpook National University, Korea.

Copyright © 2000 Korean Academy of Periodontology

Abstract

The purpose of this study is to evaluate the expression of IGF-I, considered as the mediator of action of estrogen, and IGF-IA and IGF-IB, alternative slicing form of IGF-I, using 17β-estradiol in MC3T3-E1 cells. We observed the effect on type I collagen and osteopontin gene expression and DNA synthetic activity of MC3T3-E1 cells, added by estrogen, IGF-I and combination and the interaction on proliferation and differentiation of MC3T3-E1 cells.
The results were as follows:
RT-PCR experiment for observing time-dependant IGF-I gene expression patternshowed IGF-IA and IB gene expression in both of control and test group.
In these IGF-IA gene expression was appeared predominantly. In control, IGF-I geneexpression level was maintained until 24hr and then decreased gradually. In test group, IGF-I gene expression level increased as time goes by.
Experiment measuring DNA synthetic activity, as it is added by 17β-estradiol, IGF-I and combination, showed that first day, there was the tendency of more increase of synthetic activity in all test group than control but no statical significance(P>0.05), and third day, there was more increase of DNA synthetic activity in 17β-estradiol group and combination group and it was statically significant. (P<0.005)
Experiment for observing type I collagen gene expression pattern showed more increase of expression in 17β-estradiol group than control and no significant difference in IGF-I group and combination group.
Experiment for observing osteopontin gene expression pattern showed no significant difference in control and test group.
In conclusion, 17β-estradiol in MC3T3-E1 cells increased IGF-I gene and DNA synthetic activity simultaneously, therefore it appeared that IGF-I is related to the action of estrogen. Combination treatment of IGF-I and 17β-estradiol has effect on cell proliferation but this effect is lower than IGF-I or 17β-estradiol alone. However, combination treatment has not great effect on type I collagen or osteopontin gene expression thus little effect of cell differentiation.

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