Journal List > J Korean Acad Periodontol > v.30(1) > 1049153

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Lee, Suh, Kim, and Choi: The Effects of Combination of PDGF-BB and Dexamethasone on Differentiation of MC3T3-E1 Cells
Original Article

The Journal of the Korean Academy of Periodontology 2000; 30(1): 27-37.

Published online: 31 March 2000

DOI: https://doi.org/10.5051/jkape.2000.30.1.27

The Effects of Combination of PDGF-BB and Dexamethasone on Differentiation of MC3T3-E1 Cells

Jae-mok Lee*, Jo-Young Suh*, Sung-Jo Kim**, Jeom-Il Choi**

*Department of Periodontology, School of Dentistry, Kyungpook National University, Korea.

**Department of Periodontology, School of Dentistry, Pusan National University, Korea.

Copyright © 2000 Korean Academy of Periodontology

Abstract

To evaluate the effects of Dexamethasone(Dex), Platelet derived growth factor-BB(PDGF) and combination of Dex and PDGF(DP) on the growth and differentiation of MC3T3-E1 cells, Dex(10-7 M) and PDGF(10 ng/ml) in experimental group were added to the cells at the days 5, 10, 15, 20, 25 and examined for cell proliferation activities, DNA synthesis activities, ALP activities and bone nodule formation.
The results were as follows:
1. In Dex group, cell proliferation, DNA synthesis and ALP activities were lower until 15 days when compared to the control group. Bone nodules formation were shown at 10 days.
2. In PDGF group, cell proliferation and DNA synthesis activities were higher until 15 days and ALP activities were lower when compared to the control and Dex groups. Bone nodules formation were shown at 20 days.
3. In DP group, cell proliferation and DNA synthesis activities of PDGF were suppressed by Dex and synergistic effects of combination of Dex and PDGF on ALP activities were shown at days 5 when compared to control and Dex groups. Bone nodules formation activities of Dex were suppressed by PDGF.

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