Journal List > J Korean Acad Periodontol > v.29(2) > 1049086

J Korean Acad Periodontol. 1999 Jun;29(2):349-354. Korean.
Published online June 30, 1999.
Copyright © 1999 Korean Academy of Periodontology
Development of PCR Technology for Identification of the Restriction Fragment Length Polymorphism(RFLP) of the Immunoglobulin Allotypes in Periodontal Patients
Jeom-Il Choi,* Sung-Jo Kim,* and In-Hoo Kim**
*Department of Periodontology, School of Dentistry, Pusan National University, Korea.
**Department of Biochemistry, School of Medicine, Dong-A University, Korea.

The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.

Keywords: Immunoglobulin Allotype; Restriction Fragment Length Polymorphism; Polymerase Chain Reaction