Journal List > J Korean Acad Periodontol > v.29(2) > 1049079

J Korean Acad Periodontol. 1999 Jun;29(2):277-287. Korean.
Published online June 30, 1999.
Copyright © 1999 Korean Academy of Periodontology
The Effects of Dexamethasone on Growth and Differentiation of Osteoblast-like Cell
Jae-Mok Lee
Department of Periodontology, School of Dentistry, Kyungpook National University, Korea.

The ultimate aim of periodontal treatment is periodontal regeneration, which necessiates the regeneration of bone tissues. To evaluate the effects of Dex growth and differentiation of MC3T3-E1 cells, cells were seeded in alpha-modified eagle medium containing 10% fetal bovine serum, 10mM beta-glycerophosphate, 50µg/ml of ascorbic acid, with or without 10-7M Dex and examined cell proliferation activities, alkaline phosphatase activities, and bone nodule formation until 25 days.

The results were as follows:

1. In Dex group, cell proliferation activities were lower until 15 days compared to control group. Bone nodules formation were showed at 10 days.

2. In the time-response effect, ALP activities were increased until the 10 days in control groups thereafter decreased and ALP activities of Dex group were lower aspect than control group until the 10 days.

In this study, bone nodule formation of osteoblast-like cells were accelerated by Dex and cell proliferation activities, ALP activity of Dex group showed lower than control group. Dex was considered that it did suppress initial growth, but accerelate mineralization of osteoblast-like cells.