Journal List > J Korean Acad Periodontol > v.27(4) > 1048997

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Chung, Kwon, Lee, Park, Herr, and Kim: Effect of high glucose on the prostaglandin E2 production in human gingival fibroblasts and periodontal ligament cells
Original Article

The Journal of the Korean Academy of Periodontology 1997; 27(4): 909-922.

Published online: 31 December 1997

DOI: https://doi.org/10.5051/jkape.1997.27.4.909

Effect of high glucose on the prostaglandin E2 production in human gingival fibroblasts and periodontal ligament cells

Jong-Hyuk Chung1, Young-Hyuk Kwon1, Man-Sup Lee1, Joon-Bong Park1, Yeek Herr1, Sung-Jin Kim2

1Department of Periodontology College of Dentistry, Kyung-Hee University, Korea.

2Department of Dental Pharmacology, College of Dentistry, Kyung-Hee University, Korea.

Copyright © 1997 Korean Academy of Periodontology

Abstract

The purpose of this study was to evaluate the effect of high glucose on prostaglandin E2 production in human gingival fibroblasts and periodontal ligament cells in vitro. In control group, the cells(5×104 cells/ml) were cultured with Dulbecco's Modified Eagle's Medium contained with 10% fetal bovine serum, 45mg/dl glucose. In experimental groups, glucose was added to the above culture condition at the final glucose concentrations of 100mg/dl(Test group 1), 200mg/dl (Test group 2) and 400mg/dl(Test group 3). Then each group was tested for the cell proliferation rate, protein levels, and prostaglandin E2 production at ½, 1, 2, 5 days.
The results were as follows ;

  1. As glucose concentration increased, cell proliferation rate decreased significantly at 1, 2, 5 days in human gingival fibroblasts and periodontal ligament cells(P<0.01).

  2. In human gingival fibroblasts, test group 2 and 3 showed significantly decreased protein levels as compared to control group at 5 days(P<0.01).

  3. In human periodontal ligament cells, as glucose concentration increased, protein levels decreased significantly at 2 days and 5 days(P<0.01).

  4. Prostaglandin E2 production in human gingival fibroblasts and human periodontal ligament cells significantly increased as glucose concentration increased(P<0.01).

The results at 5 days showed obvious difference as compared to those at 2 days.
From the above results, high glucose appeared to affect cellular activities including cell proliferation rate, protein levels and enhance prostaglandin E2 production. It was assumed that prostaglandin E2 production by high glucose enhances inflammatory reaction and has a toxic effect on human gingival fibroblasts and human periodontal ligament cells. This study suggests that periodontal disease in diabetic patient is related to prostaglandin E2 production.

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