Journal List > J Korean Acad Periodontol > v.27(4) > 1048983

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Lee, Lee, Choi, Yu, and Suh: The Effects of PDGF-BB on the ALP Activity of MC3T3-E1 Cells
Original Article

The Journal of the Korean Academy of Periodontology 1997; 27(4): 685-700.

Published online: 31 December 1997

DOI: https://doi.org/10.5051/jkape.1997.27.4.685

The Effects of PDGF-BB on the ALP Activity of MC3T3-E1 Cells

Kyung Hee Lee*, Jae Mok Lee*, Byung Ju Choi**, Hyun Mo Yu***, Jo Young Suh*

*Department of Periodontology, School of Dentistry, Kyungpook National University Taegu, Korea.

**Department of Periodontology, School of Dentistry, Kyungpook National University Taegu, Korea.

***Department of Oral Biochemistry, School of Dentistry, Kyungpook National University, Taegu, Korea.

Copyright © 1997 Korean Academy of Periodontology

Abstract

The ultimate aim of periodontal treatment is periodontal regeneration, which necessiates the regeneration of bone tissues. This paper investigated the effect of growth factor on bone cells. Platelet-derived growth factor(PDGF) is the one of the polypeptide growth factor that has been reported as a biological mediator which regulates activities of the cell proliferation, migration and metabolism of undifferentiated mesenchymal cells. The purpose of this study is to evaluate the effects of PDGF on bone nodule formation and ALP activity of MC3T3-E1 cells.
Cells were seeded at 1×105cells/well in alpha-modified eagle medium containing 10% fetal bovine serum, 10ml beta-glycerophosphate and 50µg/ml of ascorbic acid. PDGF 0, 0.1, 1, 10ng/ml were added to the cells at a confluent state and cultured for 3, 7, 14, 21, 28 days. We examined bone nodule formation and alkaline phosphatase activity.
The results were as follows:
There were bone nodule formation at day 21 both in control and all the experimental groups, and at day 28, all the experimental groups showed much more bone nodules than control groups.
Compared to control-1 group, ALP activity was increased in PDGF 0.1ng/ml group and was decreased in 1,10ng/ml PDGF treated groups.(P< 0.05, P< 0.01)
Compared to control-2, ALP activity was decreased in all the experimental groups except PDGF 0.1ng/ml in 21 day group.
In the time-response effect, ALP activity was increased by the day 14 in all the experimental groups and thereafter ALP activity was decreased.(P< 0.05, P< 0.01) In the dose-response effect, ALP activity was decreased as the dose of PDGF was increased, and after 21 day ALP activity was lowest in 1 ng/ml group, ALP activity was highest in the day 7 in control group and 0.1 ng/ml, 14 day experimental group.
In conclusion, PDGF is considered more effective in the proliferation than differentiation of osteoblast-like cells, and it may be useful to study the combined effect of PDGF and other growth factors on osteoblast-like cells.

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