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Park and Sung: Easy Application of Digoxigenin-11-dUTP Labelled Probe in Detection of Human Papillomavirus DNA
Original Article

Annals of Dermatology 1991; 3(2): 91-95.

Published online: 28 November 2016

DOI: https://doi.org/10.5021/ad.1991.3.2.91

Easy Application of Digoxigenin-11-dUTP Labelled Probe in Detection of Human Papillomavirus DNA

Kyang Joon Park, Ho Suk Sung

Copyright © The Korean Dermatological Association and The Korean Society for Investigative Dermatology

Abstract

In situ hybridization was performed in ten cases of condyloma acuminata in order to study the applicability of digoxigenin-11 dUTP(Dig-dUTP) labelled probe compared with radioactive isotope labelled probes.
Although signal intensity was denser in radiolabelled probes, high positive rates were obtained with Dig-dUTP labelled probes. From these results, Dig-dUTP labelling is found to be more efficient in typing of human papillomavirus DNA than radiolabelling.

Keywords: In situ hybridization, Condyloma acuminatum, Digoxigenin-11-dUTP, Radioactive isotope

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