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Choi, Kim, Choi, Kim, and Park: Translocation of p53 Protein in Melanocytes and Malignant Melanoma Cells After UVB Irradiation
Original Article

Annals of Dermatology 2001; 13(3): 133-138.

Published online: 14 March 2016

DOI: https://doi.org/10.5021/ad.2001.13.3.133

Translocation of p53 Protein in Melanocytes and Malignant Melanoma Cells After UVB Irradiation

Kyoung-Hee Choi, Hye-Jin Kim, Yong Choi, Kyu Han Kim, Kyoung-Chan Park

Copyright © The Korean Dermatological Association and The Korean Society for Investigative Dermatology

Abstract

Background

UVB is responsible for most of the carcinogenic effects of sun exposure.

Objective

Aim of this study was to investigate the regulation and intracellular redistribution of p53 protein after UVB irradiation.

Methods

Cultured normal human melanocytes and G361 melanoma cells were used for west-ern blotting and confocal microscopic examination for determining expression and distribution of p53.

Results

UVB irradiation increased p53 expression in cultured normal human melanocytes and G361 malignant melanoma cells according to increasing doses of UVB. Furthermore, p53 moved from cytosol to nucleus after UVB irradiation.

Conclusions

UVB irradiation induced overexpression and redistribution of p53 in cultured normal human melanocytes and G361 malignant melanoma cells.

Keywords: p53, UVB, translocation

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