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Park, Lim, Kim, Choi, Kim, Cho, and Youn: Caspases Activation in Ultraviolet B-induced Apoptosis of G361 Human Melanoma Cell Line
Original Article

Annals of Dermatology 2000; 12(4): 243-246.

Published online: 21 March 2016

DOI: https://doi.org/10.5021/ad.2000.12.4.243

Caspases Activation in Ultraviolet B-induced Apoptosis of G361 Human Melanoma Cell Line

Kyoung Chan Park, Jeong Gu Lim, Dong Seok Kim, Hyun Ok Choi, Kyu Han Kim, Kwang Hyun Cho, Jai Il Youn

Copyright © The Korean Dermatological Association and The Korean Society for Investigative Dermatology

Abstract

Background

Ultraviolet B (UVB) irradiation can induce apoptosis of melanocytes and melanoma cells. However, mechanism of UVB-induced apoptosis of melanoma cells is not clarified yet.

Objective

Our purpose was to study the molecular mechanism of UVB-induced apoptosis of melanoma cells.

Methods

G361 lightly pigmented melanoma cells were analyzed for apoptotic mechanism by flow cytometry and western blotting.

Results

G361 melanoma cells showed apoptotic features with gradual increment of UVB doses by MTT and flow cytometry. Western blotting disclosed activation of caspase-3 and poly (ADP-ribose) polymerase (PARP) after UVB irradiation.

Conclusion

In this study, we showed that UVB-induced apoptosis of melanoma cells is mediated by PARP activation which is induced by caspase cascade.

Keywords: Melanoma cells, Ultraviolet B, Apoptosis, Caspase

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