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Abstract
Background and Objectives
Insulin is a peptide hormone that regulates the metabolism of carbohydrates and fats by promoting the absorption of glucose from the blood to skeletal muscles. Insulin has been reported to be closely related to cardiovascular, respiratory, and endocrine disease. However, the effect of insulin on production of major mucins in human airway epithelial cells has not been reported. Therefore, this study investigated the relationship between high levels of insulin and mucin in human airway epithelial cells.
Materials and Methods
This study analyzed the effect of high level of insulin on MUC4, MUC5AC, and MUC5B expression using reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay in human airway epithelial cells.
References
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Fig. 1.
Effects of high level of insulin on MUC4 expression in NCI-H292 cells. NCI-H292 cells were pretreated with various concentration of insulin for 4hrs. RT-PCR showed that insulin significantly induced MUC4 mRNA expression in human NCI-H292 cells (A). ELISA showed that insulin significantly increased MUC4 protein production in NCI-H292 cells (B). Real-time PCR showed that the insulin-induced MUC4 mRNA expression was increased at all times and peaked at 4 h after treatment with insulin (C). The images are representative of three separate experiments performed in triplicated. Bars indicate the mean± S.D. of three independent experiments performed in triplicate. *: p＜0.05 compared with zero value. RT-PCR: reverse transcription polymerase chain reaction, ELISA: enzyme-linked immunosorbent assay.
Fig. 2.
Effects of high level of insulin on MUC5AC expression in NCI-H292 cells. NCI-H292 cells were pretreated with various concentration of insulin for 8hrs. RT-PCR showed that insulin significantly induced MUC5AC mRNA expression in human NCI-H292 cells (A). ELISA showed that insulin significantly increased MU-C5AC protein production in NCI-H292 cells (B). Real-time PCR showed that the insulin-induced MUC5AC mRNA expression was increased at all times and peaked at 8 h after treatment with insulin (C). The images are representative of three separate experiments performed in triplicated. Bars indicate the mean± S.D. of three independent experiments performed in triplicate. *: p＜0.05 compared with zero value. RT-PCR: reverse transcription polymerase chain reaction, ELISA: enzyme-linked immunosorbent assay.
Fig. 3.
Effects of high level of insulin on MUC5B expression in NCI-H292 cells. NCI-H292 cells were pretreated with various concentration of insulin for 8hrs. RT-PCR showed that insulin significantly induced MUC5B mRNA expression in human NCI-H292 cells (A). ELISA showed that insulin significantly increased MUC5B protein production in NCI-H292 cells (B). Real-time PCR showed that the insulin-induced MUC5B mRNA expression was increased at all times and peaked at 8 h after treatment with insulin (C). The images are representative of three separate experiments performed in triplicated. Bars indicate the mean± S.D. of three independent experiments performed in triplicate. *: p＜0.05 compared with zero value. RT-PCR: reverse transcription polymerase chain reaction, ELISA: enzyme-linked immunosorbent assay.
Fig. 4.
Effects of high level of insulin on MUC4, MUC5AC, MUC5B mRNA expression in human nasal epithelial cells. In human nasal epithelial cells, RT-PCR showed that insulin significantly induced MUC4, MUC5AC, and MUC5B mRNA expression (A, B, and C). The images are representative of three separate experiments performed in triplicated.
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