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Abstract
Human clonorchias is a major parasitic infection and estimated about 1.4 million people to be infected with this parasite in Korea now. Clonorchiasis is a chronic disease debilitating inhabitants and threatening their health in endemic areas. Early diagnosis and treatment of clonorchis-infections are crucial. As a standard diagnostic method, the microscopic stool examination and identification of the egg has been employed. Nowadays needs are serodiagnostic kits of more rapid, simple and convenient formats. Serodiagnostic methods using antigenic proteins, in crude or molecularly defined forms, of Clonorchis sinensis could be alternatives for diagnosis of C. sinensis-infections. Serodiagnostic methods could be employed for large scale diagnosis to screen out infected with stress on false negatives of light worm-burden cases. To alleviate these obstacles, antigenic proteins of high specificity and moderate sensitivity have been identified and available in molecularly defined forms. Recently, a rapid diagnostic test was developed for serodiagnosis of C. sinensis-infections. Multiple antigen mixtures are expected to be formulated using these defined antigenic proteins and to escalate rapid diagnostic tests to those of high specificity and sensitivity for serodiagnosis of C. sinensis-infections.
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References
1. Kim TS, Cho SH, Huh S, Kong Y, Sohn WM, Hwang SS, Chai JY, Lee SH, Park YK, Oh DK, Lee JK. A nationwide survey on the prevalence of intestinal parasitic infections in the Republic of Korea, 2004. Korean J Parasitol. 2009. 47:37–47.
2. Rim HJ. The current pathobiology and chemotherapy of clonorchiasis. Korean J Parasitol. 1986. 24:Suppl. 1–141.
3. Rim HJ. Clonorchiasis: an update. J Helminthol. 2005. 79:269–281.
4. Lee JH, Yang HM, Bak UB, Rim HJ. Promoting role of Clonorchis sinensis infection on induction of cholangiocarcinoma during two-step carcinogenesis. Korean J Parasitol. 1994. 32:13–18.
5. Choi D, Hong ST, Li S, Chung BS, Lim JH, Lee SH. Bile duct changes in rats reinfected with Clonorchis sinensis. Korean J Parasitol. 2004. 42:7–17.
6. Kim YJ, Choi MH, Hong ST, Bae YM. Resistance of cholangiocarcinoma cells to parthenolide-induced apoptosis by the excretory-secretory products of Clonorchis sinensis. Parasitol Res. 2009. 104:1011–1016.
7. Pak JH, Kim DW, Moon JH, Nam JH, Kim JH, Ju JW, Kim TS, Seo SB. Differential gene expression profiling in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory-secretory products. Parasitol Res. 2009. 104:1035–1046.
8. Sripa B, Kaewkes S, Sithithaworn P, Mairiang E, Laha T, Smout M, Pairojkul C, Bhudhisawasdi V, Tesana S, Thinkamrop B, Bethony JM, Loukas A, Brindley PJ. Liver fluke induces cholangiocarcinoma. PLoS Med. 2007. 4:e201.
9. Kim C, Kim S, Cho S. Serological reactions in early stage of experimental paragonimiasis in dog. Chung-Ang J Med. 1982. 7:335–347.
10. Soh C, Min D, Ryu J, Yong T. Study on the reproducibility of ELISA technique for the diagnosis of clonorchiasis and paragonimisasis. Yonsei Rep Trop Med. 1985. 16:1–10.
11. Kim SI. A Clonorchis sinensis-specific antigen that detects active human clonorchiasis. Korean J Parasitol. 1998. 36:37–45.
12. Shin BM, Choi K. Diagnostic Significance of Intradermal Test Compared with Radiologic Findings for Clonorchiasis. Korean J Clin Pathol. 2000. 20:81–86.
13. Yong TS, Yang HJ, Park SJ, Kim YK, Lee DH, Lee SM. Immunodiagnosis of clonorchiasis using a recombinant antigen. Korean J Parasitol. 1998. 36:183–190.
14. Yang HJ, Park SJ, Im KI, Yong TS. Identification of a Clonorchis sinensis gene encoding a vitellaria antigenic protein containing repetitive sequences. Mol Biochem Parasitol. 2000. 111:213–216.
15. Kim TY, Kang SY, Ahn IY, Cho SY, Hong SJ. Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis. Korean J Parasitol. 2001. 39:57–66.
16. Lee HJ, Lee CS, Kim BS, Joo KH, Lee JS, Kim TS, Kim HR. Purification and characterization of a 7-kDa protein from Clonorchis sinensis adult worms. J Parasitol. 2002. 88:499–504.
17. Lee M, Chung YB, Lee SK, Chung BS, Li S, Choi MH, Hong ST. The identification of a Clonorchis sinensis gene encoding an antigenic egg protein. Parasitol Res. 2005. 95:224–226.
18. Chung YB, Yang HJ, Hong SJ, Kang SY, Lee M, Kim TY, Choi MH, Chai JY, Hong ST. Molecular cloning and immunolocalization of the 17 kDa myoglobin of Clonorchis sinensis. Parasitol Res. 2003. 90:365–368.
19. Sim S, Park GM, Yong TS. Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory-secretory antigens. Parasitol Res. 2003. 91:338–343.
20. Hu F, Yu X, Ma C, Zhou H, Zhou Z, Li Y, Lu F, Xu J, Wu Z, Hu X. Clonorchis sinensis: expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-LPAP homologue. Exp Parasitol. 2007. 117:157–164.
21. Lee JS, Yong TS. Expression and cross-species reactivity of fatty acid-binding protein of Clonorchis sinensis. Parasitol Res. 2004. 93:339–343.
22. Ma C, Hu X, Hu F, Li Y, Chen X, Zhou Z, Lu F, Xu J, Wu Z, Yu X. Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis. Parasitol Res. 2007. 101:419–425.
23. Park GM, Yong TS. Geographical variation of the liver fluke, Clonorchis sinensis, from Korea and China based on the karyotypes, zymodeme and DNA sequences. Southeast Asian J Trop Med Public Health. 2001. 32:Suppl 2. 12–16.
24. Kang TH, Yun DH, Lee EH, Chung YB, Bae YA, Chung JY, Kang I, Kim J, Cho SY, Kong Y. A cathepsin F of adult Clonorchis sinensis and its phylogenetic conservation in trematodes. Parasitology. 2004. 128:195–207.
25. Na BK, Lee HJ, Cho SH, Lee HW, Cho JH, Kho WG, Lee JS, Song KJ, Park PH, Song CY, Kim TS. Expression of cysteine proteinase of Clonorchis sinensis and its use in serodiagnosis of clonorchiasis. J Parasitol. 2002. 88:1000–1006.
26. Nagano I, Pei F, Wu Z, Wu J, Cui H, Boonmars T, Takahashi Y. Molecular expression of a cysteine proteinase of Clonorchis sinensis and its application to an enzyme-linked immunosorbent assay for immunodiagnosis of clonorchiasis. Clin Diagn Lab Immunol. 2004. 11:411–416.
27. Shen C, Lee JA, Allam SR, Bae YM, Han ET, Takeo S, Tsuboi T, Hong ST, Choi MH. Serodiagnostic applicability of recombinant antigens of Clonorchis sinensis expressed by wheat germ cell-free protein synthesis system. Diagn Microbiol Infect Dis. 2009. 64:334–339.
28. Kang SY, Ahn IY, Park CY, Chung YB, Hong ST, Kong Y, Cho SY, Hong SJ. Clonorchis sinensis: molecular cloning and characterization of 28-kDa glutathione S-transferase. Exp Parasitol. 2001. 97:186–195.
29. Hong SJ, Yun Kim T, Gan XX, Shen LY, Sukontason K, Kang SY. Clonorchis sinensis: glutathione S-transferase as a serodiagnostic antigen for detecting IgG and IgE antibodies. Exp Parasitol. 2002. 101:231–233.
30. Chandler J, Gurmin R, Robinson N. The place of gold in rapid tests. IVD Technology. 2000. 37–43.
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