The Effect of Supplementary Selenium on Leukocytes and HSP70 Expression after Half-Body Immersion

Young-Oh Shin; Min-Kyu Han; Jeong-Beom Lee; Byung-Hun Um

doi:10.4163/kjn.2011.44.5.378

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Shin, Han, Lee, and Um: The Effect of Supplementary Selenium on Leukocytes and HSP70 Expression after Half-Body Immersion

Research Article

Korean Journal of Nutrition

Korean Journal of Nutrition 2011; 44(5): 378-383.

Published online: 31 October 2011

DOI: https://doi.org/10.4163/kjn.2011.44.5.378

The Effect of Supplementary Selenium on Leukocytes and HSP70 Expression after Half-Body Immersion

Young-Oh Shin¹, Min-Kyu Han¹, Jeong-Beom Lee², Byung-Hun Um³

¹Department of Health Care, Graduate School, Soonchunhyang University, Asan 336-745, Korea.

²Department of Physiology, College of Medicine, Soonchunhyang University, Chonan 331-946, Korea.

³Functional Food Center, KIST Gangneung 210-340, Korea.

To whom correspondence should be addressed. (leejb@sch.ac.kr)

Received 12 July 2011 Revised 9 August 2011 Accepted 16 September 2011

Abstract

This study examined the effect of supplementary selenium on leukocytes and heat shock protein (HSP) 70 expression in serum during half-body immersion. The subjects were male college tennis athletes. All subjects participated in two repeated experiments with a 1 week interval. During the 30 min intermittent half-body immersion, subjects were given 500 mL of water with or without selenium (100 µg). Blood samples were taken from the antecubital vein, and differential counts were made. Serum HSP70 protein was analyzed using a commercial ELISA kit. After half-body immersion, leukocytes and lymphocytes increased significantly but neutrophils decreased significantly in both trials (with or without selenium). Selenium supplementation, compared with placebo, decreased levels of leukocytes, neutrophils, and monocytes, but not lymphocytes, to the resting level or below 60 min after immersion. Only lymphocytes continued to increase in both trials during the recovery period. Serum HSP70 protein level did not change after immersion, but it decreased 60 min after immersion with the administration of selenium. In conclusion, supplementary selenium reduced the systemic immune response and serum HSP70 protein accumulation after half-body immersion.

Keywords: half-body immersion, selenium, serum, HSP70, leukocytes

Figures and Tables

Fig. 1

Changes of leukocytes and subsets over time in two treatments. White bar: water and placebo, black bar: water and selenium.

^*: p < 0.05, ^**: p <0.01, ^***: p <0.001; compare with Pre in each treatment.

Fig. 2

HSP70 expression over time in two treatments. White bar: water and placebo, black bar: water and selenium. ^*: p <0.05. compare with Pre in each treatment.

Table 1

Characteristics of subjects

Mean ± SD

Table 2

Changes of leukocytes and HSP70 protein over time in two treatments

Mean ± SD

^*: p < 0.05, ^**: p<0.01, ^***: p < 0.001; compare with Pre in each treatment

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