Journal List > Korean J Nutr > v.42(5) > 1043771

Zhang, Choi, and Seo: Effect of Dietary Grape Pomace on Lipid Oxidation and Related Enzyme Activities in Rats Fed High Fat Diet


The present study was conducted to investigate the effect of dietary supplementation of grape pomace on lipid peroxidation and related enzyme activities of rats fed high fat diet. Male Sprague-Dawley rats weighing about 90 g were assigned to 4 experimental groups of 8 rats on the basis of their body weight. The high fat diet contained additional 15% lard to AIN 93-based diet. Rats were fed experimental diets containing 5% grape pomace for 4 weeks. Dietary supplementation of grape pomace reduced serum concentration of lipid peroxide in rats fed high fat diet. Hepatic concentration of lipid peroxide tended to be lower by feeding grape pomace. Hepatic total glutathione content and GSH/GSSG ratio were increased by grape pomace feeding in normal or high fat diet groups. Hepatic superoxide dismutase activity of grape pomace group with high fat diet was induced significantly compared with high fat diet group without grape pomace. Hepatic catalase activity of high fat fed rats was induced by feeding grape pomace. Grape pomace diet increased glutathione-S-transferase and glutathione peroxidase activities in rat liver fed high fat. Hepatic glucose-6-phosphatase activity was not affected by dietary supplementation of grape pomace in rats fed high fat. These results suggest that dietary supplementation of grape pomace may alleviate lipid peroxidation through antioxidant effect in rats fed high fat.

Figures and Tables

Fig. 1
Effect of grape pomace diet on serum concentration of lipid peroxide in rats fed normal or high fat diet. Means ± S.D. Values with the same superscript letter are not significantly different (p < 0.05).
Fig. 2
Effect of grape pomace diet on hepatic homogenate (A) and microsomal (B) concentration of lipid peroxide in rats fed normal or high fat diet. Means ± S.D. Values with the same superscript letter are not significantly different (p < 0.05).
Fig. 3
Effect of grape pomace diet on hepatic level of total glutathione (A) and liver GSH/GSSG ratio (B) in rats fed normal or high fat diet. Means ± S.D. Values with the same superscript letter are not significantly different (p < 0.05).
Table 1
Composition of experimental diet

1) Mineral mixture according to AIN-93 (Teklad, USA)

2) Vitamin mixture according to AIN-93 (Teklad, USA)

C: Control diet, CP: Control diet + Pomace, HF: High fat diet, HFP: High fat diet + Pomace

Table 2
Effect of grape pomace diet on food intake, weight gain and feed efficiency ratio in rate fed normal or high fat diet

1) Groups are the same as the Table 1

2) mean ± SD. Values with the same superscript letter within the column are not significantly different (p < 0.05)

FER: Feed Efficiency Ratio, NS: not siginificant

Table 3
Effect of grape pomace diet on SOD, catalase, G6Pase, GST and GSH-Px activities in liver fraction of rats fed normal or high fat diet (unit/min/mg protein)

Means ± S.D. Values with the same superscript letter within the column are not significantly different (p < 0.05)


This research was supported by 2005 grant from Agricultural R & D Promotion Center (ARPC).


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