Quantitative analysis of mutans streptococci adhesion to various orthodontic bracket materials in vivo

Jin-Kyoung Yu; Sug-Joon Ahn; Shin-Jae Lee; Young-Il Chang

doi:10.4041/kjod.2009.39.2.105

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Yu, Ahn, Lee, and Chang: Quantitative analysis of mutans streptococci adhesion to various orthodontic bracket materials in vivo

Original Article

Korean Journal of Orthodontics

Korean Journal of Orthodontics 2009; 39(2): 105-111.

Published online: 30 April 2009

DOI: https://doi.org/10.4041/kjod.2009.39.2.105

Quantitative analysis of mutans streptococci adhesion to various orthodontic bracket materials in vivo

Jin-Kyoung Yu, BS^a, Sug-Joon Ahn, DDS, MSD, PhD^b, Shin-Jae Lee, DDS, MSD, PhD^c, Young-Il Chang, DDS, MSD, PhD^d

^aGraduate student, School of Dentistry, Seoul National University, Korea.

^bAssistant Professor, Department of Orthodontics and DRI, School of Dentistry, Seoul National University, Korea.

^cAssociate Professor, Department of Orthodontics and DRI, School of Dentistry, Seoul National University, Korea.

^dProfessor, Department of Orthodontics and DRI, School of Dentistry, Seoul National University, Korea.

Corresponding author: Sug-Joon Ahn. Department of Orthodontics, School of Dentistry, Seoul National University, 28, Yeongeon-dong, Jongno-gu, Seoul 110-749, Korea. +82 2 2072 2672; titoo@snu.ac.kr

Received 7 May 2008 Revised 15 December 2008 Accepted 20 December 2008

Abstract

Objective

To estimate the effects of bracket material type on enamel decalcification during orthodontic treatment, this study analyzed the adhesion level of mutans streptococci (MS) to orthodontic bracket materials in vivo.

Methods

Three different types of orthodontic bracket materials were used: stainless steel, monocrystalline sapphire, and polycrystalline alumina. A balanced complete block design was used to exclude the effect of positional variation of bracket materials in the oral cavity. Three types of plastic individual trays were made and one subject placed the tray in the mouth for 12 hours. Then, the attached bacteria were isolated and incubated on a mitis salivarius media containing bacitracin for 48 hours. Finally, the number of colony forming units of MS was counted. The experiments were independently performed 5 times with each of the 3 trays, resulting in a total of 15 times. Mixed model ANOVA was used to compare the adhesion amount of MS.

Results

There was no difference in colony forming units among the bracket materials irrespective of jaw and tooth position.

Conclusions

This study suggested that the result of quantitative analysis of MS adhesion to various orthodontic bracket materials in vivo may differ from that of the condition in vitro.

Keywords: Orthodontic bracket, Mutans streptococci, Bacterial adhesion, in vivo experiment

Figures and Tables

Fig 1

Three sets of the plastic individual trays on which 3 types of bracket raw materials were attached in a different order.

Table 1

Amount of mutans streptococci adhesion isolated from the surface of bracket raw materials attached on the plastic individual trays. The counting unit was based on the colony forming unit per ml (cfu/ml)

SD, Standard deviation.

References

1. Margolis MJ. Esthetic considerations in orthodontic treatment of adults. Dent Clin North Am. 1997. 41:29–48.

2. Nattrass C, Sandy JR. Adult orthodontics--a review. Br J Orthod. 1995. 22:331–337.

3. Gorelick L, Geiger AM, Gwinnett AJ. Incidence of white spot formation after bonding and banding. Am J Orthod. 1982. 81:93–98.

4. Artun J, Brobakken BO. Prevalence of carious white spots after orthodontic treatment with multibonded appliances. Eur J Orthod. 1986. 8:229–234.

5. Gwinnett AJ, Ceen RF. Plaque distribution on bonded brackets: a scanning microscope study. Am J Orthod. 1979. 75:667–677.

6. Ahn SJ, Lim BS, Lee SJ. Prevalence of cariogenic streptococci on incisor brackets detected by polymerase chain reaction. Am J Orthod Dentofacial Orthop. 2007. 131:736–741.

7. Ulukapi H, Koray F, Efes B. Monitoring the caries risk of orthodontic patients. Quintessence Int. 1997. 28:27–29.

8. Ahn SJ, Lim BS, Yang HC, Chang YI. Quantitative analysis of the adhesion of cariogenic streptococci to orthodontic metal brackets. Angle Orthod. 2005. 75:666–671.

9. Ahn SJ, Kho HS, Kim KK, Nahm DS. Adhesion of oral streptococci to experimental bracket pellicles from glandular saliva. Am J Orthod Dentofacial Orthop. 2003. 124:198–205.

10. Ahn SJ, Lee SJ, Baek SH, Kim TW, Chang YI, Nahm DS, et al. Direct detection of cariogenic streptococci in metal brackets in vivo using polymerase chain reaction. Korean J Orthod. 2005. 35:312–319.

11. Kim M, Lim SA. Identification of mutans streptococci Isolated from Dental Plaque Formed between Bracket and Tooth Surface in Orthodontic Patients. 2003. 782:In : 81st General Session of the International Association for Dental Research; 351.

12. Whiley RA, Beighton D. Current classification of the oral streptococci. Oral Microbiol Immunol. 1998. 13:195–216.

13. Sansone C, Van Houte J, Joshipura K, Kent R, Margolis HC. The association of mutans streptococci and non-mutans streptococci capable of acidogenesis at a low pH with dental caries on enamel and root surfaces. J Dent Res. 1993. 72:508–516.

14. Ahn SJ, Lee SJ, Lim BS, Nahm DS. Quantitative determination of adhesion patterns of cariogenic streptococci to various orthodontic brackets. Am J Orthod Dentofacial Orthop. 2007. 132:815–821.

15. Fournier A, Payant L, Bouclin R. Adherence of Streptococcus mutans to orthodontic brackets. Am J Orthod Dentofacial Orthop. 1998. 114:414–417.

16. Papaioannou W, Gizani S, Nassika M, Kontou E, Nakou M. Adhesion of Streptococcus mutans to different types of brackets. Angle Orthod. 2007. 77:1090–1095.

17. Lim BS, Lee SJ, Lee JW, Ahn SJ. Quantitative analysis of adhesion of cariogenic streptococci to orthodontic raw materials. Am J Orthod Dentofacial Orthop. 2008. 133:882–888.

18. Ahn SJ, Ihm JA, Nahm DS. Adherence of salivary proteins to various orthodontic brackets. Korean J Orthod. 2002. 32:443–453.

19. Anhoury P, Nathanson D, Hughes CV, Socransky S, Feres M, Chou LL. Microbial profile on metallic and ceramic bracket materials. Angle Orthod. 2002. 72:338–343.

20. Christersson CE, Dunford RG, Glantz PO, Baier RE. Effect of critical surface tension on retention of oral microorganisms. Scand J Dent Res. 1989. 97:247–256.

21. Eliades T, Eliades G, Brantley WA. Microbial attachment on orthodontic appliances: I. Wettability and early pellicle formation on bracket materials. Am J Orthod Dentofacial Orthop. 1995. 108:351–360.

22. Busscher HJ, Doornbusch GI, Van der Mei HC. Adhesion of mutants streptococci to glass with and without a salivary coating as studied in a parallel-plate flow chamber. J Dent Res. 1992. 71:491–500.

23. Lee SJ, Kho HS, Lee SW, Yang WS. Experimental salivary pellicles on the surface of orthodontic materials. Am J Orthod Dentofacial Orthop. 2001. 119:59–66.

24. Brantley WA, Eliades T. Orthodontic materials: scientific and clinical aspects. 2001. 1st ed. Stuttgart: Thieme;142–161.

25. Pratt-Terpstra IH, Weerkamp AH, Busscher HJ. On a relation between interfacial free energy-dependent and noninterfacial free energy-dependent adherence of oral streptococci to solid substrata. Curr Microbiol. 1988. 16:311–313.

26. Quirynen M, Marechal M, Busscher HJ, Weerkamp AH, Darius PL, van Steenberghe D. The influence of surface free energy and surface roughness on early plaque formation. An in vivo study in man. J Clin Periodontol. 1990. 17:138–144.

27. Quirynen M, Bollen CM. The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man. A review of the literature. J Clin Periodontol. 1995. 22:1–14.

28. Barsamian-Wunsch P, Park JH, Watson MR, Tinanoff N, Minah GE. Microbiological screening for cariogenic bacteria in children 9 to 36 months of age. Pediatr Dent. 2004. 26:231–239.

29. Yang YM, Kim SS, Jun ES, Park SB. Changes of periodontopathogens and clinical parameters of periodontal tissue after debanding. Korean J Orthod. 2006. 36:263–274.

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