The effects of ipriflavone on the periodontal reorganization following experimental tooth movement in the rat

Ji-Hyun Min; Jin-Hyoung Cho; Ki-Heon Lee; Hyeon-Shik Hwang

doi:10.4041/kjod.2008.38.5.347

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Min, Cho, Lee, and Hwang: The effects of ipriflavone on the periodontal reorganization following experimental tooth movement in the rat

Original Article

Korean Journal of Orthodontics

Korean Journal of Orthodontics 2008; 38(5): 347-357.

Published online: 30 October 2008

DOI: https://doi.org/10.4041/kjod.2008.38.5.347

The effects of ipriflavone on the periodontal reorganization following experimental tooth movement in the rat

Ji-Hyun Min, DDS, MSD^a, Jin-Hyoung Cho, DDS, MSD, PhD^b, Ki-Heon Lee, DDS, MSD, PhD^c, Hyeon-Shik Hwang, DDS, MSD, PhD^d

^aGraduate Student, Department of Orthodontics, School of Dentistry, Chonnam National University, Korea.

^bAssistant Professor, Department of Orthodontics, 2nd Stage of Brain Korea 21, School of Dentistry, Dental Science Research Institute, Chonnam National University, Korea.

^cAssociate Professor, Department of Orthodontics, 2nd Stage of Brain Korea 21, School of Dentistry, Dental Science Research Institute, Chonnam National University, Korea.

^dProfessor, Department of Orthodontics, 2nd Stage of Brain Korea 21, School of Dentistry, Dental Science Research Institute, Chonnam National University, Korea.

Corresponding author: Hyeon-Shik Hwang. Department of Orthodontics, Chonnam National University Hospital, Hak-Dong 8, Dong-Gu, Gwangju 501-757, Korea. +82 62 220 5486; hhwang@chonnam.ac.kr

Received 24 August 2007 Revised 23 January 2008 Accepted 26 January 2008

Abstract

Objective

The purpose of this study was to examine the effect of ipriflavone on periodontal reorganization and prevention of relapse following tooth movement.

Methods

Orthodontic rubber bands were inserted between the first and second maxillary molars of 27 white male rats for 3 weeks for experimental tooth movement. From one day before the removal of orthodontic rubber band, ipriflavone was administered 50 or 400 mg/kg daily in each experimental group whereas carboxymethyl cellulose solution was administered in the control group. They were sacrificed at the 5, 10, and 15th day from the day of removal of orthodontic rubber bands. The amount of relapse was evaluated by measuring the interdental space, and the extent of periodontal reorganization was compared through histological examination.

Results

In case of ipriflavone administration, the amount and velocity of relapse was less and slower compared to the control group. In addition, the ipriflavone group showed more rapid periodontal reorganization compared to the control group.

Conclusions

The results of the present study suggest that ipriflavone administration can be used effectively in the prevention of relapse following orthodontic tooth movement through the acceleration of periodontal reorganization.

Keywords: Ipriflavone, Periodontal reorganization, Relapse, Experimental tooth movement

Figures and Tables

Fig. 1

Chemical structure of ipriflavone (7-isopropoxy-3-phenyl-4H-1-benzopyran-4-one) used in this study.

Fig. 2

Time line diagram of experimental strategy.

Fig. 3

Change of interdental spaces according to time elapsed. The change of interdendal space was slow and gradual in ipriflavone treated groups while steep decrease was observed in control group. Most of changes occurred at 5 days in control group.

Fig. 4

The extent of periodontal reorganization was compared through the histologic examination. A, Normal alveolar bone and periodontal ligament before tooth movement are shown; B, the control group right after the removal of latex elastic band. Irregular alveolar crest and disintegrated or disorientated periodontal fibers are shown; C, D, E, the control group at day 5, 10, 15 after the removal of latex elastic band; F, G, H, the experimental group of ipriflavone treatment (50 mg) at day 5, 10, 15 after the removal of latex elastic band; I, J, K, the experimental group of ipriflavone treatment (400 mg) at day 5, 10, 15 after the removal of latex elastic band. In experimental groups, the periodontal reorganization occurred more rapidly and the margin of alveolar bone showed more smooth appearance compared to the control group; tf, transseptal fiber; ob, osteoblast; af, alveolar crest fiber; oc, osteoclast; od, osteoid; pf, periodontal fiber; bt, bone trabeculae.

Table 1

Interdental spaces between the first and second molars in control group and ipriflavone treated groups according to time elapsed (unit: mm)

Values are mean (standard deviation); n = 6. IP 50, ipriflavone 50 mg; IP 400, ipriflavone 400 mg; ^*p < 0.05; ^†p < 0.01; NS, not significant.

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