Journal List > Korean J Pediatr Gastroenterol Nutr > v.14(Suppl 1) > 1043539

Bae: Stem Cell in Pediatric Gastrointestinal Tract Disease: Hirschsprung Disease


Stem cell is characterized with self-renewal and mult-potency. Many pediatric gastrointestinal diseases have defect in enterocytes, enteric nervous system, and Interstitial cell of Cajal. Various kinds of stem cell could be applied to these diseases. Here, the author introduces stem cell for pediatric gastrointestinal diseases, particularly Hirschsprung disease.

Figures and Tables

Fig. 1
Lineage tracing in the small intestine confirms intestinal stem cells. In this case, LacZ activity was used as a reporter gene8).
Fig. 2
Neurons from transplanted neurospheres regulate the contractility of developing gut. EMNS and NHNS transplanted bowel have comparable contraction frequencies to ganglionic distal colon, but have significantly lower contraction frequencies than aganglionic distal colon, demonstrating a functional effect of neurosphere transplantation18). EMNS: aganglionic distal colon transplanted with embryonic mouse neurosphere, NHNS: aganglionic distal colon transplanted with neonatal human neurosphere, TTX: tetrodotoxin.


1. Quante M, Wang TC. Stem cell in gastroenterology and hepatology. Nat Rev Gastroenterol Hepatol. 2009. 6:724–737.
2. Mills JC, Shivdasani RA. Gastric epitheilial stem cell. Gastroenterology. 2011. 140:412–424.
3. Malgieri A, Kantzari E, Patrizi MP, Gambardella S. Bone marrow and umbilical cord blood human mesenchymal stem cells: state of the art. Int J Clin Exp Med. 2010. 3:248–269.
4. Barker N, De Wetering MV, Clvers H. The intestinal stem cell. Genes Dev. 2008. 22:1856–1864.
5. Takeda N, Jain R, LeBoeuf MR, Wang Q, Lu MM, Epstein JA. Interconversion between intestinal stem cell populations in distinct niches. Science. 2011. 334:1420–1424.
6. Sato T, Van Es JH, Snippert HJ, Stange DE, Vries RG, van den Born M, et al. Paneth cells constitute the niche for Lgr5 stem cells in the intestinal crypts. Nature. 2011. 469:415–418.
7. Kelly PN, Dakic A, Adams M, Nutt SL, Strasser A. Tumor growth need not be driven by rare cancer stem cell. Science. 2007. 317:337.
8. Snippert HJ, Van Es JV, van den Born M, Begthel H, Stange DE, Barker N, et al. promin-1/CD133 marks stem cells and early progenitors in mouse small intestine. Gastroenterology. 2009. 136:2187–2194.e1.
9. Umar S. Intestinal stem cells. Curr Gastroenterol Rep. 2010. 12:340–348.
10. Meirelles Lda S, Fontes AM, Covas DT, Caplan AI. Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev. 2009. 20:419–427.
11. Shi Y, Hu G, Su J, Li W, Chen Q, Shou P, et al. Mesenchymal stem cells: a new strategy for immunosuppression and tissue repair. Cell Research. 2010. 20:510–518.
12. Kuo TK, Hung SP, Chuang CH, Chen CT, Shih YR, Fang SC, et al. Stem cell therapy for liver disease: parameters governing the success of using bone marrow mesenchymal stem cells. Gastroenterology. 2008. 134:2111–2121.
13. Brittan M, Alison MR, Schier S, Wright NA. Bone marrow stem cell-mediated regeneration in IBD: where do we go from here? Gastroenterology. 2007. 132:1171–1173.
14. Kapur RP. Hirschsprung disease and other enteric dysgangliosis. Crit Rev Clin Lab Sci. 1999. 36:225–273.
15. Almond S, Lindley RM, Kenny SE, Connell MG, Edgar DH. Characterisation and transplantation of enteric nervous system progenitor cells. Gut. 2007. 56:489–496.
16. Metzger M, Bareiss PM, Danker T, Wagner S, Hennelotter J, Guenther E, et al. Expansion and differentiation of neural progenitors derived from the human adult enteric nervous system. Gastroenterology. 2009. 137:2063–2073.
17. Metzger M, Cadwell C, Barlow AJ, Burns AJ, Thapar N. Enteric nervous system stem cells derived from human gut mucosa for the treatment of aganglionic gut disorders. Gastroenterology. 2009. 136:2214–2225.
18. Lindley RM, Hawcutt DB, Connell MG, Almond SN, Vannucchi MG, Simonetta M, et al. Human and mouse enteric nervous system neurosphere transplants regulate the function of aganglionic embryonic distal colon. Gastroenterology. 2008. 135:205–216.
Similar articles