Min Sung Cho; Seung Min Song; Seak Hee Oh; Yeoun Joo Lee; Ju Young Jang; Kyung Mo Kim

doi:10.5223/kjpgn.2011.14.3.269

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Cho, Song, Oh, Lee, Jang, and Kim: Tumor Necrosis Factor-α Gene Polymorphisms in Korean Children with Inflammatory Bowel Disease

Original Article

Gastrointestinal Tract

Korean Journal of Pediatric Gastroenterology and Nutrition

Korean Journal of Pediatric Gastroenterology and Nutrition 2011; 14(3): 269-278.

Published online: 30 September 2011

DOI: https://doi.org/10.5223/kjpgn.2011.14.3.269

Tumor Necrosis Factor-α Gene Polymorphisms in Korean Children with Inflammatory Bowel Disease

Min Sung Cho, M.D., Seung Min Song, M.D., Seak Hee Oh, M.D., Yeoun Joo Lee, M.D., Ju Young Jang, M.D., Kyung Mo Kim, M.D.

Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea.

Corresponding author (kmkim@amc.seoul.kr)

Received 30 August 2011 Revised 18 September 2011 Accepted 20 September 2011

Abstract

Purpose

The aim of this study was to investigate the existence of TNF-α polymorphisms in Korean children with Crohn's disease (CD), ulcerative colitis (UC), as compared to healthy controls.

Methods

Blood samples were obtained from 40 patients with CD, 14 patients with UC, and 30 healthy controls. Genotyping for 5 TNF-α polymorphisms (G238A, G308A, C857T, C863A, and T1031C) was performed. The allele frequencies for the inflammatory bowel diseases, CD and UC, were measured in patients with these disease and in healthy controls, and these allele frequencies were compared between the 3 groups. We examined the significant association between polymorphism and disease phenotype, such as location, behavior, perianal disease, and pediatric Crohn's activity index (PCDAI) in CD.

Results

Based on our findings, the TNF-α allele frequencies of G238A, G308A, C857T, C863A, and T1031C were 3.3, 8.3, 16.7, 16.7, 21.7% in healthy control, 2.5%, 7.5%, 18.8%, 20.0%, 22.5% in CD, 7.1%, 7.1%, 7.1%, 21.4%, 28.6% in UC. They were no statistically significant differences between the 3 groups. There were no associations between genotypes and phenotypes in CD, except a statistically significant higher allele frequency of G238A in ileal type (L1) disease (p=0.010).

Conclusion

Our results indicate that 5 TNF-α polymorphisms do not seem to be associated with susceptibility to inflammatory bowel disease in Korean pediatric patients even though young patients were anticipated to have a stronger genetic affiliation for these diseases than adult patients. We think that further studies are needed to find those genes associated with susceptibility to CD and UC in Korean pediatric patients with inflammatory bowel disease.

Keywords: Crohn's disease, TNF-α, Gene polymorphism, Ulcerative colitis

Figures and Tables

Table 1

Oligonucleotide Primers Used for Genomic PCR (all sequences are listed 5' to 3')

Table 2

Characteristics of the Patients with CD, UC and Healthy Controls

UC: ulcerative colitis, CD: Crohn's disease, HC: healthy Control, PCDAI: Pediatric Crohn's disease activity index.

Table 3

Genotype and Allele Frequencies of Polymorphism in the TNF-α Gene

Table 4

Correlation Based on the Location and Genotype and Allele Frequencies of Polymorphism in the TNF-α Gene

Table 5

Correlation Based on the Behavior and Genotype and Allele Frequencies of Polymorphism in the TNF-α Gene

Table 6

Correlation Based on the Perinatal Lesion and Genotype and Allele Frequencies of Polymorphism in the TNF-α Gene

Table 7

Correlation Based on the PCDAI and Genotype and Allele Frequencies of Polymorphism in the TNF-α Gene

Table 8

TNF Haplotype Frequency Estimates in Controls and Crohn's Disease

^*The SNP positions within a haplotype are the following:-1,031C>T, -863C>A, -857C>T, -308G>A, -238G>A.

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