Journal List > Korean J Pediatr Gastroenterol Nutr > v.14(3) > 1043511

Korean J Pediatr Gastroenterol Nutr. 2011 Sep;14(3):258-268. Korean.
Published online September 30, 2011.  https://doi.org/10.5223/kjpgn.2011.14.3.258
Copyright © 2011 The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition
FOXP3+T Cells and TGF-β1 in Colonic Mucosa of Children with Crohn's Disease
Joo Hyun Gil, M.D., Jung Eun Oh, M.D., Jeong Wan Seo, M.D., Min-Sun Cho, M.D.,* Ky Young Cho, M.D. and Eun Sun Yoo, M.D.
Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea.
*Department of Pathology, Ewha Womans University School of Medicine, Seoul, Korea.

Corresponding author (Email: jwseo@ewha.ac.kr )
Received February 27, 2011; Revised March 15, 2011; Accepted March 17, 2011.

Abstract

Purpose

Forkhead box protein 3 (FOXP3)+T cells are the major regulatory T cells controlling all aspects of the immune response. Transforming growth factor-β (TGF-β) is a suppressive cytokine which mediates the suppressive action of FOXP3+T cells. The aim of this study was to investigate the role of FOXP3+T cells, TGF-β in colonic mucosa of children with Crohn's disease (CD).

Methods

Colonic mucosal biopsies were obtained from 10 children with CD (12~15 years of age) and 11 control (8~15 years of age). Frequencies of FOXP3+T, CD4+T cells and TGF-β1 expression were examined in the lamina propria (LP) and lymphoid aggregates or follicles (LA/F) by immunohistochemistry, and later evaluated by association with disease activity.

Results

In the LP of CD group, frequencies of FOXP3+T, CD4+T cells, proportion of FOXP3/CD4+T cells and TGF-β1 expression significantly increased compared to the control. In the LA/F of CD group, frequency of FOXP3+T cells, proportion of FOXP3/CD4+T cells and TGF-β1 expression significantly increased compared to the control (p<0.05). CD4+T cells also increased compared to the control, but this finding was not significant. In the LP and LA/F of CD group, frequency of FOXP3+T cells exhibited positive correlation with CD4+T cells (p<0.05). In the LP and LA/F of CD group, TGF-β1 expression had positive correlation with CRP, Pediatric Crohn's Disease Activity Index, and negative correlation with hematocrit and albumin (p<0.05).

Conclusion

Increased frequency of FOXP3+T cells and TGF-β1 expression in colonic mucosa of CD can be interpreted as a compensatory increase towards achieving down-regulation of immune responses.

Keywords: T-lymphocytes; Regulatory; Crohn's disease; FOXP3; CD4; Transforming growth factor beta; Child; Disease activity

Figures


Fig. 1
Immunohistochemical staining of FOXP3 (×400). (A) In the lamina propria (LP) of Crohn's disease (CD) group. (B) In the LP of the control. (C) In the lymphoid aggregates or follicles (LA/F) of CD group. (D) In the LA/F of the control.
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Fig. 2
Immunohistochemical staining of CD4 (×400). (A) In the LP of CD group. (B) In the LP of the control. (C) In the LA/F of CD group. (D) In the LA/F of the control.
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Fig. 3
Immunohistochemical staining of TGF-β1 (×400). (A) Grade 0 in the control, (B) Grade 1, (C) Grade 2, (D) Grade 3 in CD group.
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Fig. 4
The frequencies of FOXP3+T, CD4+T cells, and the proportion of FOXP3+T cells to CD4+T cells and TGF-β1 expression were more increased in the lamina propria of Crohn's disease group (n=10) than the control (n=11). (A) FOXP3+T cells, (B) CD4+T cells, (C) FOXP3/CD4+T cells, (D) TGF-β1 expression.
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Fig. 5
The frequencies of FOXP3+T, CD4+T cells, and the proportion of FOXP3+T cells to CD4+T cells and TGF-β1 expression were more increased in the lymphoid aggregates or follicles of Crohn's disease group (n=8) than the control (n=10). (A) FOXP3+T cells, (B) CD4+T cells, (C) FOXP3/CD4+T cells, (D) TGF-β1 expression.
Click for larger image

Tables


Table 1
Demographic and Clinical Characteristics of Patients with Crohn's Disease and Control Subjects
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Table 2
Correlation of the Frequencies of FOXP3+T Cells with the Frequencies of CD4+T Cells, TGF-β1 Expression in CD Group
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Table 3
Correlation of the Frequencies of FOXP3+T, CD4+T Cells, Expression of TGF-β1 with Disease Activity Parameters in CD Group
Click for larger image

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