Analysis of Gene Expression in Helicobacter pylori-associated Nodular Gastritis in Children Using Microarray

Hye Ran Yang; Jae Sung Ko; Jeong Kee Seo

doi:10.5223/kjpgn.2010.13.1.7

Journal List > Korean J Pediatr Gastroenterol Nutr > v.13(1) > 1043481

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Yang, Ko, and Seo: Analysis of Gene Expression in Helicobacter pylori-associated Nodular Gastritis in Children Using Microarray

Original Article

Gastrointestinal Tract

Korean Journal of Pediatric Gastroenterology and Nutrition

Korean Journal of Pediatric Gastroenterology and Nutrition 2010; 13(1): 7-22.

Published online: 31 March 2010

DOI: https://doi.org/10.5223/kjpgn.2010.13.1.7

Analysis of Gene Expression in Helicobacter pylori-associated Nodular Gastritis in Children Using Microarray

Hye Ran Yang, M.D., Jae Sung Ko, M.D., Jeong Kee Seo, M.D.

Division of Gastroenterology and Nutrition, Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea.

Responsible author (jkseo@snu.ac.kr)

Received 7 February 2010 Accepted 6 March 2010

Abstract

Purpose

Nodular gastritis is a characteristic finding of Helicobacter pylori infection in children. The aim of this study was to evaluate the difference in gene expression in the gastric mucosa of H. pylori-infected and non-infected children, and to analyze the difference in gene expression using cDNA microarray analysis of nodular gastritis caused by H. pylori infection.

Methods

Twelve children (6 boys and 6 girls; mean age 9.8 years) who underwent upper gastrointestinal endoscopy and biopsy at Seoul National University Bundang Hospital were included in the study. The subjects were divided into three groups according to the presence of H. pylori infection and nodular gastritis on endoscopic examination. Gastric mucosa tissue was kept at -70℃ and RNA was extracted to perform cDNA microarray analysis in each patient.

Results

cDNA microarray analysis in children revealed a clear distinction between H. pylori-infected and non-infected gastric mucosa. Specifically, 182 over-expressed genes and 29 under-expressed genes were identified in H. pylori-infected gastric mucosa compared to non-infected mucosa. H. pylori-infected nodular gastritis revealed different gene expression patterns from H. pylori-infected normal gastric mucosa; five genes were over-expressed and five genes were under-expressed.

Conclusion

In the presence of H. pylori infection, gastric mucosa shows distinct differences in gene expression, and nodular gastritis with H. pylori infection in children may be associated with over- or under-expression of some genes. Further studies are required to clarify the host response and the pathogenesis of nodular gastritis in children.

Keywords: Helicobacter pylori, Nodular gastritis, cDNA microarray, Gene expression, Children

Figures and Tables

Fig. 1

Hierarchical clustering of 37,351 genes expressed in the gastric mucosa of children in 3 groups based on the presence of Helicobacter pylori infection (HP) and nodular gastritis (NG). The red color indicates over-expressed genes and the green color indicates under-expressed genes.

Table 1

List of Genes Over-expressed in the Gastric Mucosa of Helicobacter pylori-infected Children Compared to Non-infected Gastric Mucosa

Table 2

List of Genes Under-expressed in the Gastric Mucosa of Helicobacter pylori-infected Children Compared to Non-infected Gastric Mucosa

Table 3

List of Genes Over- or Under-expressed in the Gastric Mucosa of Children with Helicobacter pylori-associated Nodular Gastritis Compared to Normal-appearing Helicobacter pylori-infected Gastric Mucosa

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