Journal List > J Korean Foot Ankle Soc > v.22(1) > 1043459

Park, Cho, Lee, Woo, and Lim: In Vitro Study Evaluating the Antimicrobial Activity of Vancomycin-Impregnated Cement Stored at Room Temperature in Methicillin-Resistant Staphylococcus aureus

Abstract

Purpose:

Treatment of diabetic foot infection due to methicillin-resistant Staphylococcus aureus (MRSA) remains challenging. Applying vancomycin-impregnated cement is one of the best methods of treatment. Vancomycin-impregnated cement has been used worldwide; however, to date, there is a limited number of studies regarding its use. We evaluated the duration of antimicrobial activity of vancomycin-impregnated cement stored at room temperature after manufacturing.

Materials and Methods:

The vancomycin-impregnated cement was manufactured by mixing 1 g of vancomycin with 40 g of polymer and adding 17.90 g of liquid monomer. The cement dough was shaped into flat cylinders with diameter and height of 6 mm and 2 mm, respectively. Another cement of the same shape without mixing vancomycin was prepared as the negative control. All manufactured cements were sterilized with ethylene oxide gas and stored at room temperature. Each cement was placed on Mueller Hinton agar plate lawned with standard MRSA strain. Standard vancomycin disk and gentamicin disk were placed together. After 24 hours, the diameter of inhibition zone was measured, and if the diameter was less than 15 mm, vancomycin-impregnated cement was regarded as a loss of antimicrobial activity. The study was repeated every 2 weeks until vancomycin-impregnated cements lost their antimicrobial activity.

Results:

Vancomycin-impregnated cement stored for a duration of 16 weeks created a 14 mm inhibition zone, while vancomycin disk created a 15 mm inhibition zone. Vancomycin-impregnated cement stored for a duration of 17 weeks created 7 mm and 9 mm inhibition zones, while vancomycin disk created 16 mm and 15 mm inhibition zones, respectively.

Conclusion:

We found a decrease of antimicrobial activity in vancomycin-impregnated cements after 16 weeks. After 17 weeks, they showed definite loss of antimicrobial activity. Therefore, we recommend not using vancomycin-impregnated cement spacers that has been stored for more than 16 weeks at room temperature.

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Figure 1.
The antibiotics-impregnated cements were manufactured as a disk form 6 mm diameter and 2 mm height.
jkfas-22-38f1.tif
Figure 2.
The vancomycin-impregnated cement and the gentamicin-impregnated cement were placed on Mueller Hinton agar plate lawned with methicillin-resistant Staphylococcus aureus. Commercially available vancomycin disk and gentamicin disk were placed on the same plate. Each cement and disk was placed with at least 24 mm distance.
jkfas-22-38f2.tif
Figure 3.
In every agar plate, the gentamicin-impregnated cements (*) and gentamicin disks () as negative control didn’t create inhibition zones while vancomycin disks () as positive control created inhibition zones after 24 hours incubation which were larger than 15 mm wide.
jkfas-22-38f3.tif
Figure 4.
The vancomycin-impregnated cement, vancomycin disk and gentamicin disk were placed on the Mueller Hinton agar plate lawned with methicillin-resistant Staphylococcus aureus (A) and incubated for 24 hours. The vancomycin-impregnated cement stored for 17 weeks (B) created 7 mm inhibition zone while vancomycin disk as positive control (C) created 16 mm inhibition zone after 24 hours incubation.
jkfas-22-38f4.tif
Figure 5.
The vancomycin-impregnated cement, vancomycin disk and gentamicin disk were placed on the Mueller Hinton agar plate lawned with methicillin-resistant Staphylococcus aureus (A) and incubated for 24 hours. The vancomycin-impregnated cement stored for 17 weeks (B) created 9 mm inhibition zone while vancomycin disk as positive control (C) created 15 mm inhibition zone after 24 hours incubation.
jkfas-22-38f5.tif
Table 1.
The Inhibition Zone Diameter Depending on the Storage Periods
Weeks
0 2 4 6 8 10 12 14 16 17
VA antibead1 19 18 15 18 15 19 18 16 14 7
VA control1 19 18 18 19 19 19 19 18 15 16
GM control1 0 0 0 0 0 0 0 0 0 0
VA antibead2 19 18 18 18 15 16 16 16 16 9
VA control2 19 18 18 19 19 19 19 20 16 15
GM control2 0 0 0 0 0 0 0 0 0 0
GM antibead1 0 0 0 0 0 0 0 0 0 0
VA control1 20 19 19 19 20 19 19 20 18 19
GM control1 0 0 0 0 0 0 0 0 0 0
GM antibead2 0 0 0 0 0 0 0 0 0 0
VA control2 19 20 20 19 20 19 18 18 19 18
GM control2 0 0 0 0 0 0 0 0 0 0

VA antibead: vancomycin-impregnated cement, VA control: vancomycin disk, GM control: gentamicin disk, GM antibead: gentamicin-impregnated cement.

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