Journal List > Korean J Phys Anthropol > v.14(1) > 1038793

Korean J Phys Anthropol. 2001 Mar;14(1):89-96. Korean.
Published online April 14, 2017.  https://doi.org/10.11637/kjpa.2001.14.1.89
Copyright © 2001 Korean Association of Physical Anthropologists
Effect of Inorganic Polyphosphate on Cultured Periodontal Ligament Cells
Dong Ju Lee and Je Won Shin
Department of Oral Anatomy, College of Dentistry, Kyung Hee University, Korea.
Abstract

This study was designed to examine the effect of polyP on the periodontal ligament fibroblasts. The cells were incubated for 24 and 48 hours with various concentrations (0.001%, 0.002%, 0.005%, 0.010%, 0.020%, 0.040%) of polyP. Cell activity, alkaline phosphatase activity, and protein synthesis activity of the cells were examined. The results were as follows: 1. Twenty four hours after incubation with polyP, the cell activity of the periodontal ligament fibroblasts was increased. Forty eight hours after polyP application, the activity of the cells was similar to that of control group. 2. Twenty four or forty eight hours after incubation with polyP, the alkaline phosphatase activity of the periodontal ligament fibroblasts was increased. 3. The protein synthesis of the periodontal ligament fibroblasts was not changed after polyP application. These results suggest that, polyP may increase the cell activity and alkaline phosphatase activity of periodotal ligament fibroblasts and polyP may not affect the protein synthesis of the cells.

Keywords: polyP; Periodontal ligamental fibroblast; ALP activity; Protein synthesis; MTT assay