Comparison of SD BIOLINE Rapid Influenza Antigen Test Using Two Different Specimens, Nasopharyngeal Swabs and Nasopharyngeal Aspirates

Hae In Bang; Jeong Won Shin; Tae Youn Choi; Rojin Park; Yu Jeong Shin

doi:10.5145/KJCM.2010.13.4.147

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Bang, Shin, Choi, Park, and Shin: Comparison of SD BIOLINE Rapid Influenza Antigen Test Using Two Different Specimens, Nasopharyngeal Swabs and Nasopharyngeal Aspirates

Original Article

Korean J Clin Microbiol 2010;13(4):147-150.

Published online: 21 December 2010

DOI: https://doi.org/10.5145/KJCM.2010.13.4.147

Comparison of SD BIOLINE Rapid Influenza Antigen Test Using Two Different Specimens, Nasopharyngeal Swabs and Nasopharyngeal Aspirates

Hae In Bang, Jeong Won Shin, Tae Youn Choi, Rojin Park, Yu Jeong Shin

Department of Laboratory Medicine, Soonchunhyang University College of Medicine, Seoul, Korea

Correspondence: Tae Youn Choi, Department of Laboratory Medicine, Soonchunhyang University Hospital, 22 Daesagwan-gil, Yongsan-gu, Seoul 140-743, Korea. (Tel) 82-2-709-9425, (Fax) 82-2-709-9083, (E-mail) choity@hosp.sch.ac.kr

Received 31 May 2010 Revised 14 July 2010 Accepted 20 August 2010

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

The pandemic swine origin influenza A/H1N1 2009 virus (H1N1 2009) was rapidly spread out all over the world after it was first found in April, 2009. This study was made to compare the performance of nasopharyngeal swabs and nasopharyngeal aspirates for the SD Bioline rapid influenza antigen test.

Methods

From Aug to Nov, 2009 the SD Bioline rapid influenza antigen tests were conducted with the nasopharyngeal swabs and the nasopharyngeal aspirates from the 244 specimens of patients who had come to the hospital with influenza-like illness. The data from the examination were compared with the multiplex RT-PCR as a reference standard to obtain sensitivity, specificity, positive predictive value and negative predictive value.

Results

The sensitivity and the specificity of the SD Bioline rapid influenza antigen tests with the nasopharyngeal swabs were 75.8%, and 93.3% respectively, and the sensitivity and specificity with the nasopharyngeal aspirates were 61.3%, and 98.3% respectively.

Conclusion

Even if the nasopharyngeal aspirates showed the lower sensitivity than the nasopharyngeal swabs, since the specificity is higher, the nasopharyngeal aspirates are more useful because we can reduce false positive rate.

Keywords: H1N1, Rapid influenza antigen test, Multiplex RT-PCR

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Table 1.

Performance of SD BIOLINE RAT in comparison with multiplex RT-PCR using two different specimens, nasopharyngeal swabs and nasopharyngeal aspirates

Specimens	No. of case				% sensitivity (95% CI)	% specificity (95% CI)	% PPV (95% CI)	% NPV (95% CI)
Specimens	PCR+ RAT+	PCR+ RAT−	PCR− RAT+	PCR− RAT−	% sensitivity (95% CI)	% specificity (95% CI)	% PPV (95% CI)	% NPV (95% CI)
NPS	94	30	8	112	75.8 (70.4∼81.2)	93.3 (90.2∼96.4)	92.2 (88.8∼95.6)	78.9 (73.8∼84.0)
NPA	76	48	2	118	61.3 (55.2∼67.4)	98.3 (96.7∼99.9)	97.4 (95.4∼99.4)	71.1 (65.4∼76.8)

Abbreviations: RAT, rapid antigen test; PPV, positive predictive value; NPV, negative predictive value; NPS, nasopharyngeal swab; NPA, nasopharyngeal aspirate.

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