Journal List > Korean J Clin Microbiol > v.15(3) > 1038275

Yim, Hwang, Kim, Lim, Shin, Chung, Kim, and Lee: Evaluation of a ChromID C. difficile Agar for the Isolation of Clostridium difficile

Abstract

Background

Clostridium difficile is the main etiologic agent of antibiotic-associated diarrhea and the most common cause of hospital-acquired diarrhea. Recently, the incidence of C. difficile infections (CDI) has increased and new highly virulent C. difficile strains have emerged. Therefore, accurate and rapid diagnosis is needed. We compared the results of using chromID C. difficile (chromID CD, bioMeriéux, France) with the conventional C. difficile Selective Agar (CDSA; BD, USA) for the isolation of C. difficile.

Methods

A total of 738 stool specimens of suspected CDI patients at the Severance Hospital from July to August 2011 were inoculated onto CDSA. Among them, 104 stool specimens revealed colonies on CDSA that were then re-inoculated onto chromID CD. The stool samples were stored at -20℃ until the time of the re-inoculation. Cultured agars were interpreted after 24 hrs and 48 hrs, respectively. Species identification was performed on the basis of colony characteristics on agar plates as well as the ATB 32A system (API System SA, France).

Results

The recovery rates of CDSA and chromID CD were 30.1% and 77.5% after 24 hrs, and 77.5% and 98.6% after 48 hrs, respectively. All of the C. difficile isolates were recovered as typical gray/black colonies on chromID CD.

Conclusion

The performance of chromID CD for the isolation of C. difficile was better than that of conventional CDSA. The chromID CD could provide easy and sensitive detection of C. difficile even after 24hrs of incubation.

Figures and Tables

Fig. 1
Typical colonies of Clostridium difficile on chromID CD.
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Table 1
Recovery rate (%) of Clostridium difficile on CDSA and chromID CD
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*C. difficile recovered from 71 specimens.

Abbreviations: CDSA, Clostridium difficile Selective Agar; ChromID CD, chromID C. difficile agar.

Table 2
Non-Clostridium difficile isolates recovered on chromID CD from 104 stool samples
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Abbreviation: chromID CD, chromID C. difficile agar.

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