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Park, Ki, and Lee: Laboratory Experience in Phenotypic and Molecular Identification of Blastomyces dermatitidis First Isolated in Korea
Letter to the Editor
Korean Journal of Clinical Microbiology

Korean Journal of Clinical Microbiology 2012; 15(3): 114-116.

Published online: 20 September 2012

DOI: https://doi.org/10.5145/KJCM.2012.15.3.114

Laboratory Experience in Phenotypic and Molecular Identification of Blastomyces dermatitidis First Isolated in Korea

Kyung Sun Park, Chang-Seok Ki, Nam Yong Lee

Department of Laboratory Medicine and Genetics, Sungkyunkwan University School of Medicine, Seoul, Korea.

Correspondence: Nam Yong Lee, Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50, Irwon-dong, Gangnam-gu, Seoul 135-710, Korea. (Tel) 82-2-3410-2706, (Fax) 82-2-3410-2719, micro.lee@samsung.com

Copyright © 2012 The Korean Society of Clinical Microbiology

Abstract

Blastomycosis, endemic in North America, has been hardly reported in Korea. We describe laboratory experience in phenotypic and molecular identification of Blastomyces dermatitidis first isolated in Korea. The patient was a 45-year-old male with pulmonary blastomycosis mimicking pulmonary tuberculosis. Diagnosis was based on culture and dimorphism combined with DNA target sequencing of internal transcribed spacers (ITS) and D1/D2 regions.

Keywords: Blastomyces dermatitidis, Blastomycosis, D1/D2, Internal transcribed spacers (ITS), Molecular identification

Figures and Tables

Fig. 1
(A) White, cottony colonies on the front side of Sabouraud's dextrose agar (incubated at 30℃ for 10 days). (B) Tan, wrinkled colonies on the reverse side of Sabouraud's dextrose agar (incubated at 30℃ for 10 days). (C) Septate hyphae with round, lollipop-like conidia which are developing at the apex of the conidiophores (incubated at 30℃ for 10 days, lactophenol cotton blue stain, ×400).
kjcm-15-114-g001
Fig. 2
(A) Cream-colored colonies on brain heart infusion (BHI) agar (incubated at 35℃ for 12 days). (B) Yeastlike cells with characteristic thick, double contoured walls and broad-based buds (incubated at 35℃ for 12 days, lactophenol cotton blue stain, ×400).
kjcm-15-114-g002

References

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5. Pappas PG. Blastomycosis in the immunocompromised patient. Semin Respir Infect. 1997. 12:243–251.
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