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Yoon, Hong, Lee, Chang, and Hong: A Case of a 63-bp Deletion in the mpt64 Gene of Mycobacterium tuberculosis Strains Which Showed False Negativity in the Immunochromatographic Assay
Original Article

Korean J Clin Microbiol 2011;14(1):36-38.

Published online: 21 March 2011

DOI: https://doi.org/10.5145/KJCM.2011.14.1.36

A Case of a 63-bp Deletion in the mpt64 Gene of Mycobacterium tuberculosis Strains Which Showed False Negativity in the Immunochromatographic Assay

Chang Eun Yoon, Young Joon Hong, Jin Kyung Lee, Yoon Hwan Chang, Seok-Il Hong

Department of Laboratory Medicine, Korea Cancer Center Hospital, Seoul, Korea

Correspondence: Seok-Il Hong, Department of Laboratory Medicine, Korea Cancer Center Hospital, 215-4 Gongneung 2-dong, Nowon-gu, Seoul 139-706, Korea. (Tel) 82-2-970-2492, (Fax) 82-2-973-7143, (E-mail) hongsicp@kcch.re.kr

Received 4 May 2010       Revised 28 June 2010       Accepted 20 July 2010

Copyright © 2011 Korean Journal of Clinical Microbiology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mycobacterium tuberculosis complex (MTBC) is discriminated from non-tuberculous mycobacteria (NTM) via an immunochromatographic assay (ICA) which is based on the reactions of monoclonal antibodies against MPT64, one of the predominant proteins excreted by MTBC. Recently, the authors of the present study discovered SD TB-negative Mycobacterium tuberculosis strains. In addition, sequence analysis of the mpt64 genes in these strains was performed and showed a deletion of 63 bp from nucleotides 196 to 258. In cases of MPT64-negative mycobacterium, the authors recommend performing TB PCR for correct diagnosis.

Keywords: Mycobacterium tuberculosis, mpt64 gene, Mutation

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