Journal List > Korean J Clin Microbiol > v.13(3) > 1038190

Korean J Clin Microbiol. 2010 Sep;13(3):121-124. Korean.
Published online September 30, 2010.
Copyright © 2010 The Korean Society of Clinical Microbiology
Importance of Suspicion for the Identification of Mycoplasma in Wound Culture: A Case Report
Sang Mee Hwang, In Seon Yoon, Sei-Ick Joo, Jongyoun Yi and Eui-Chong Kim
Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea.

Correspondence: Eui-Chong Kim, Department of Laboratory Medicine, College of Medicine, Seoul National University, 101 Daehang-ro, Jongno-gu, Seoul 110-744, Korea. (Tel) 82-2-2072-3500, (Fax) 82-2-747-0359, Email:
Received January 21, 2010; Revised July 01, 2010; Accepted July 20, 2010.


Genital mycoplasmas are rare in extraintestinal specimens, but can cause disseminated infections in immunocompromised patients and wound infections after surgery or injury. We report two cases of Myoplasma hominis wound infections after lung lobectomy and kidney transplantation, and a case of M. salivarium wound infection after aortic graft replacement. Mycoplasmas grew in aerobic and anaerobic cultures as tiny colonies but were not observed by gram- or acid fast stain and were confirmed by MYCOFAST EvolutioN 2 kit or 16S rRNA sequencing. These cases indicated that mycoplasmas were probably underestimated in wound infections because they were not in suspicion. We suggest that Mycoplasma should be suspected when microorganisms are not readily observable in Gram stains but can be cultured.

Keywords: Mycoplasma hominis; Mycoplasma salivarium; Wound infection

1. Miranda C, Camacho E, Reina G, Turiño J, Rodríguez-Granger J, Yeste R, et al. Isolation of Mycoplasma hominis from extragenital cultures. Eur J Clin Microbiol Infect Dis 2005;24:334–337.
2. Taylor-Robinson D. The role of mycoplasmas in pregnancy outcome. Best Pract Res Clin Obstet Gynaecol 2007;21:425–438.
3. Lee JH, Lee JH, Lee NY, Ha CW, Chung DR, Peck KR. Two cases of septic arthritis by Mycoplasma hominis after total knee replacement arthroplasty. Korean J Lab Med 2009;29:135–139.
4. Madoff S, Hooper DC. Nongenitourinary infections caused by Mycoplasma hominis in adults. Rev Infect Dis 1988;10:602–613.
5. Engel LD. Mycoplasma salivarium in human gingival sulci. J Periodontal Res 1970;5:163–171.
6. Grisold AJ, Hoenigl M, Leitner E, Jakse K, Feierl G, Raggam RB, et al. Submasseteric abscess caused by Mycoplasma salivarium infection. J Clin Microbiol 2008;46:3860–3862.
7. So AK, Furr PM, Taylor-Robinson D, Webster AD. Arthritis caused by Mycoplasma salivarium in hypogammaglobulinaemia. Br Med J (Clin Res Ed) 1983;286:762–763.
8. Meyer RD, Clough W. Extragenital Mycoplasma hominis infections in adults: emphasis on immunosuppression. Clin Infect Dis 1993;17 Suppl 1:S243–S249.
9. Mattila PS, Carlson P, Sivonen A, Savola J, Luosto R, Salo J, et al. Life-threatening Mycoplasma hominis mediastinitis. Clin Infect Dis 1999;29:1529–1537.
10. Miranda C, Carazo C, Bañón R, Mendoza J, Montes A, de la Rosa M. Mycoplasma hominis infection in three renal transplant patients. Diagn Microbiol Infect Dis 1990;13:329–331.
11. Sielaff TD, Everett JE, Shumway SJ, Wahoff DC, Bolman RM 3rd, Dunn DL. Mycoplasma hominis infections occurring in cardiovascular surgical patients. Ann Thorac Surg 1996;61:99–103.
12. Park HR, Kim YH, Lee HJ, Oh JS, Kim HJ. Usefulness of the mycofast test (MYCOFAST(R) Evolution 2) for the diagnosis of nongonococcal genitourinary infections. Korean J Urol 2006;47:1117–1123.
13. Bayraktar MR, Ozerol IH, Gucluer N, Celik O. Prevalence and antibiotic susceptibility of Mycoplasma hominis and Ureaplasma urealyticum in pregnant women. Int J Infect Dis 2010;14:e90–e95.
14. Petrikkos GL, Hadjisoteriou M, Daikos GL. PCR versus culture in the detection of vaginal Ureaplasma urealyticum and Mycoplasma hominis. Int J Gynaecol Obstet 2007;97:202–203.