A Case of Granulicatella adiacens Septicemia Identified by 16S rRNA Sequencing Analysis

Kyeong Seob Shin; Bo Ra Son; Hye Won Jeong

doi:10.5145/kjcm.2008.11.1.63

Journal List > Korean J Clin Microbiol > v.11(1) > 1038151

Go to TopGo to Top Go to BottomGo to Bottom

TOOLS

Shin, Son, and Jeong: A Case of Granulicatella adiacens Septicemia Identified by 16S rRNA Sequencing Analysis

Original Article

Korean J Clin Microbiol 2008;11(1):63-65.

Published online: 20 April 2008

DOI: https://doi.org/10.5145/kjcm.2008.11.1.63

A Case of Granulicatella adiacens Septicemia Identified by 16S rRNA Sequencing Analysis

Kyeong Seob Shin^1,, Bo Ra Son¹, Hye Won Jeong²

¹Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju, Korea

²Department of Internal Medicine, College of Medicine, Chungbuk National University, Cheongju, Korea

Correspondence: Kyeong Seob Shin, Department of Laboratory Medicine, College of Medicine, Chungbuk National University, 62, Gaesin-dong, Heungduk-gu, Cheongju 361-711, Korea. (Tel) 82-43-269-6240, (Fax) 82-43-271-5243, (E-mail) ksshin@chungbuk.ac.kr

Received 2 January 2008 Accepted 26 February 2008

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Granulicatella adiacens is one of the fastidious gram positive cocci previously described as nutritionally variant streptococci due to their requirement of L-cysteine, pyridoxal, or thiol compounds for growth. These bacteria have been identified as significant causative agents of endocarditis, opthalmic infections, and meningitis. We report a case of septicemia caused by G. adiacens in an 80-year-old patient with cholangiocarcinoma. The organism was identified by pheno-typic and 16S rRNA sequencing analyses.

Keywords: Nutritionally variant streptococci, Granuli-catella adiacens, Septicemia

References

1. Bouvet A, Grimont F, Grimont PAD. Streptococcus defectives sp. nov. and Streptococcus adiacens sp. nov., nutritionally variant streptococci from human clinical specimens. Int J Syst Bacteriol. 1989; 39:290–4.

2. Carey RB, Gross KC, Roberts RB. Vitamin B6-dependent Streptococcus mitior (mitis) isolated from patients with systemic infections. J Infect Dis. 1975; 131:722–6.

3. Roberts RB, Krieger AG, Schiller NL, Gross KC. Viridans streptococcal endocarditis; the role of various species, including pyridoxal-dependent streptococci. Rev Infect Dis. 1979; 1:955–65.

4. Frenkel A and Hirsch W. Spontaneous development of L forms of streptococci requiring secretions of other bacteria or sulphydryl compounds for normal growth. Nature (London). 1961; 191:728–30.

5. George RH. The isolation of symbiotic streptococci. J Med Microbiol. 1974; 7:77–83.

6. Namdari H, Kintner K, Jacksons BA, Namdari S, Hughes L, Peairs RR, et al. Abiotrophia species as a cause of endophthalmitis following cataract extraction. J Clin Microbiol. 1999; 37:1564–6.

7. Biermann C, Fries G, Jehnichen P, Bhakdi S, Husmann M, Isolation of Abiotrophia adiacens from a brain abscess which developed in a patient after neurosurgery. J Clin Microbiol. 1999; 37:769–71.

8. Lee SY and Pai CH. Two cases of nutritionally variant streptococci from patients with endocarditis. Korean J Clin Pathol. 1995; 15:273–80.

9. Kwon O, Park SD, Uh Y, Yoon KJ, Kwon SO, Kim HY. A case of septicemia by Granulicatella adiacens. Infect Chemother. 2005; 37:368–71.

10. Thornsberry C, Swenson JM, Baker CN, McDougal LK, Stocker SA, Hill BC. Methods for determining susceptibility of fastidious and unusual pathogens to selected antimicrobial agents. Diagn Microbiol Infect Dis. 1988; 9:139–53.

11. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement. CLSI document M100-S16, Wayne, PA; CLSI. 2006.

12. Kawamura Y, Hou XG, Sultana F, Liu S, Yamamoto H, Ezaki T. Transfer of Streptococcus adiacens and Streptococcus defectives to Abiotrophia gen. nov. as Abiotrophia adiacens comb. nov. and Abiotrophia defectives comb. nov., respectively. Int J Syst Bacteriol. 1995; 45:798–803.

13. Collins MD and Lawson PA. The genus Abiotrophia (Kawamura et al.) is not monophyletic; propose of Granulocatella gen. nov., Granulicatella adiacens comb. nov., Granulicatella elegans comb. nov. and Granulicatella balaenopterae comb. nov. Int J Syst Evol Microbiol. 2000; 50:365–9.

14. Carey RB, Brause BD, Roberts RB. Antimicrobial therapy of vitamin B6-dependent streptococcal endocarditis. Ann Intern Med. 1977; 87:150–4.

15. Tuohy MJ, Procop GW, Washington JA. Antimicrobial susceptibility of Abiotrophia adiacens and Abiotrophia defectiva. Diagn Microbiol Infect Dis. 2000; 38:189–91.

Fig. 1.

Gram stain of Granulicatella adiacens isolated from the case. Smears were prepared directly from a positive blood culture vial (Bact/Alert 3D blood culture broth) (A), or from a culture grown again in the blood culture broth supplemented with 0.001% pyridoxal hydrochloride (B). Figure A shows characteristic gram-positive pleomorphic cocci in pairs and short chains. In addition yeast like cells were noted. Figure B shows characteristic gram-positive cocci in typical streptococcal chains.

TOOLS

Similar articles