Journal List > J Korean Gastric Cancer Assoc > v.5(4) > 1037004

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Lee, Cho, Song, Park, Nam, Lee, Yoo, Lee, and Park: Mutation of the Chk1 Gene in Gastric Cancers with Microsatellite Instability
Original Article

Journal of the Korean Gastric Cancer Association 2005; 5(4): 260-265.

Published online: 13 March 2017

DOI: https://doi.org/10.5230/jkgca.2005.5.4.260

Mutation of the Chk1 Gene in Gastric Cancers with Microsatellite Instability

Jong Heun Lee, Young Gu Cho, Jae Whie Song, Cho Hyun Park1, Suk Woo Nam, Sug Hyung Lee, Nam Jin Yoo, Jung Young Lee, Won Sang Park

Department of Pathology, The Catholic University of Korea College of Medicine, Seoul, Korea.

1Department of Surgery, The Catholic University of Korea College of Medicine, Seoul, Korea.

Corresponding author (wonsang@catholic.ac.kr)

Copyrights © 2005 by The Korean Gastric Cancer Association

Abstract

Purpose

The protein kinase Chk1 is required for cell cycle arrest in response to DNA damage and is shown to play an important role in the G2/M checkpoint. The aim of this study was to investigate the relationship between microsatellite instability and frameshift mutation of the Chk1 gene in gastric cancers.

Materials and Methods

The microsatellite instability was analyzed in 95 primary gastric carcinomas by using microdissection and 6 microsatellite markers. We also performed single strand conformational polymorphism and sequencing to detect frameshift mutation of the Chk1 gene.

Results

We found positive microsatellite instability in 19 (20%) of the 95 gastric cancers, 13 high- and 6 low-frequency microsatellite instability cases. The frameshift mutation of Chk1, which resulted in a truncated Chk1 protein, was detected in two high-frequency microsatellite instability cases.

Conclusion

These data suggest that the microsatellite instability may contribute to the development of gastric carcinomas through inactivation of Chk1.

Keywords: Gastric cancer, Microsatellite instability, Mutation, Cell cycle

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ORCID iDs

Won Sang Park
https://orcid.org/http://orcid.org/0000-0002-9090-0584

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