Journal List > J Korean Gastric Cancer Assoc > v.4(4) > 1036963

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Yoo, Lee, Soung, Jeon, Nam, Kim, Park, Lee, and Lee: Mutational Analysis of the Epidermal Growth Factor Receptor Gene in Gastrointestinal Stromal Tumors
Original Article

Journal of the Korean Gastric Cancer Association 2004; 4(4): 268-271.

Published online: 13 March 2017

DOI: https://doi.org/10.5230/jkgca.2004.4.4.268

Mutational Analysis of the Epidermal Growth Factor Receptor Gene in Gastrointestinal Stromal Tumors

Nam Jin Yoo1, Jong Woo Lee1, Young Hwa Soung1, Hae Myung Jeon2, Suk Woo Nam1, Su Young Kim1, Won Sang Park1, Jung Young Lee1, Sug Hyung Lee1

1Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.

2Department of General Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Corresponding author (suhulee@catholic.ac.kr)

Copyrights © 2004 by The Korean Gastric Cancer Association

Abstract

Purpose

Most gastrointestinal stromal tumors (GISTs) have gain-of-function mutations of the KIT or the platelet-derived growth factor receptor alpha (PDGFRA) genes, but approximately 10% of the GISTs are wild types for both the KIT and the PDGFRA genes. The purpose of this study was to investigate the possibility that epidermal growth factor receptor (EGFR) gene mutation might be responsible for the pathogenesis of GIST.

Materials and Methods

We analyzed the EGFR gene in 60 GISTs for the detection of somatic mutations by using the polymerase chain reaction (PCR), the single strand conformation polymorphism (SSCP), and DNA sequencing in exon 18, 19, and 21 encoding the kinase domain.

Results

The SSCP analysis revealed no evidence of EGFR mutations in exon 18, 19, and 21 in GISTs.

Conclusion

The data indicate that the EGFR gene may not be mutated in human GIST and suggest that therapies targeting the mutated EGFR gene products might not be useful in the treatment of GISTs.

Keywords: Epidermal growth factor receptor gene, Gastrointestinal stromal tumor, Mutation

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ORCID iDs

Won Sang Park
https://orcid.org/http://orcid.org/0000-0002-9090-0584

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