Abstract
Purpose
Methods
Results
Figures and Tables
Figure 1
Expression analyses of UCK2 gene in patients with breast cancer. (A) UCK2 expression in breast cancer tissues relative to controls from the Oncomine database. Analyses showing p-values <10−5 and fold change values >|2| are marked in red. The size of the circle is scaled by the sample size of the corresponding analysis. (B) One array study E-TABM-276 comparing UCK2 expression in breast tissues from patients with breast cancer to UCK2 expression in breast tissues from healthy controls was identified in the Array Express database. The median normalized log2 expression values of UCK2 were 3.6 in breast tissues from healthy controls, 5.6 in tumor adjacent normal tissues, 6.4 in tissues exhibiting cystic change, and 7.4 in invasive breast cancer tissues. *Indicated p-value less than 0.05.
![jbc-20-132-g001](/upload/SynapseData/ArticleImage/0096jbc/jbc-20-132-g001.jpg)
Figure 2
Association of UCK2 gene expression with tumor characteristics. UCK2 gene expression was associated with molecular subtypes (A), tumor grade (B) and estrogen receptor (ER) status (C) in patients with breast cancer using the GOBO (Gene Expression-based Outcome for Breast Cancer Online) database. The top row each figure indicates the number of patients with breast cancer in the analyses.
![jbc-20-132-g002](/upload/SynapseData/ArticleImage/0096jbc/jbc-20-132-g002.jpg)
Figure 3
Enriched gene signatures associated with aggressiveness and prognosis in UCK2-high and -low breast cancers. Normalized enrichment score (NES) represents the NES for the gene-set enrichment analyses. The ranked list metric was generated by calculating the signal-to-noise ratio, which is based on the difference of means scaled according to the standard deviation. The larger the signal-to-noise ratio, the more distinct the gene expression is for each phenotype. The heat maps show the enrichment of genes in the gene sets. Columns are individual samples and rows represent each gene. Blue indicates a low level of expression, and red indicates a high level of expression. (A, B) are the examples showing that UCK2 expression levels were inversely associated with breast cancer prognosis, and (C) shows the genes coexpressed with UCK2 were enriched in biological pathways associated with tumor grade, molecular subtype, cancer invasiveness, metastasis, and prognosis.
![jbc-20-132-g003](/upload/SynapseData/ArticleImage/0096jbc/jbc-20-132-g003.jpg)
Figure 4
The prognostic significance of UCK2 in breast cancer. Multivariable Cox regression analyses of UCK2 with overall survival (OS) (A) and disease-free survival (DFS) (B) in patients with adjustment for age, grade and estrogen receptor (ER) status. Cox regression analyses of UCK2 with OS in patients with ER− (C) and ER+ (D) breast cancer, respectively. Multivariable analyses of UCK2 with DFS in patients with ER− (E) and ER+ (F) breast cancer, respectively. Hazard ratios (HRs) and 95% confidence intervals (CIs) were adjusted for age and grade for (C–F).
![jbc-20-132-g004](/upload/SynapseData/ArticleImage/0096jbc/jbc-20-132-g004.jpg)
Figure 5
The prognostic performances of UCK2 expression, the 70-gene signature, the wound-response gene signature, the 21-gene recurrence score, and the TNM staging in patients with estrogen receptor (ER)+ breast cancer in the NKI dataset using multivariable Cox proportional hazards regression models. (A) Overall survival (OS).(B) Disease-free survival (DFS).
![jbc-20-132-g005](/upload/SynapseData/ArticleImage/0096jbc/jbc-20-132-g005.jpg)
Table 1
Cox-proportional hazards regression analyses of UCK2 gene expression and breast cancer survival in 10 public microarray datasets
![jbc-20-132-i001](/upload/SynapseData/ArticleImage/0096jbc/jbc-20-132-i001.jpg)
HR=hazard ratio; CI=confidence interval; NA=not applicable.
*For multivariate analyses, HR was adjusted by age, estrogen receptor (ER) status, and Elston grade in GSE10885, GSE25066, GSE53031, GSE22226; for GSE1456, it was adjusted by Elston grade, ER and human epidermal growth factor receptor 2 status; for GSE2034, HR was adjusted by ER status. For GSE4922 and GSE7390, HR was adjusted by age, ER, Elston grade T stage and N stage. For GSE58812, HR was adjusted by age and ER. The NKI set was adjusted by age, grade, ER, tumor size, and lymph node status; †Pooled analysis was performed with adjustment for age, grade and ER status.
ACKNOWLEDGMENTS
References
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