Expression of Cyclin D1 and bcl-2 in Infiltrative Ductal Carcinoma of the Breast: Their Correlations and Clinical implications

Young Ki Hong; Seung Sam Baik; Min Sung Chung; Ho Sung Yoon

doi:10.4048/jbc.2008.11.4.172

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Hong, Baik, Chung, and Yoon: Expression of Cyclin D1 and bcl-2 in Infiltrative Ductal Carcinoma of the Breast: Their Correlations and Clinical implications

Original Article

Journal of Breast Cancer

Journal of Breast Cancer 2008; 11(4): 172-179.

Published online: 31 December 2008

DOI: https://doi.org/10.4048/jbc.2008.11.4.172

Expression of Cyclin D1 and bcl-2 in Infiltrative Ductal Carcinoma of the Breast: Their Correlations and Clinical implications

Young Ki Hong, Seung Sam Baik¹, Min Sung Chung, Ho Sung Yoon

Department of Surgery, Hanyang University Hospital, College of Medicine, Seoul, Korea.

¹Department of Pathology, Hanyang University Hospital, College of Medicine, Seoul, Korea.

acts123@hanmail.net

Received 5 September 2008 Accepted 5 November 2008

Abstract

Purpose

Cyclin D1 and bcl-2 are involved in cell proliferation and apoptosis in tumor development and are commonly expressed in breast cancer. But there are few clinical reports on the correlation between cyclin D1 and bcl-2 expression. This study was designed to analyze the correlations of cyclin D1 and bcl-2 and their clinical implications in breast cancer.

Methods

Immunohistochemical expression of cyclin D1 and bcl-2 were studied in 342 infiltrative ductal carcinoma cases and were compared with clinicopathologic parameters such as age, tumor size, histologic grade, lymph node status, p53, c-erbB2 and hormone receptors.

Results

Cyclin D1 expression was found in 86 of 342 cases (25.1%). Bcl-2 was positive in 227 of 342 cases (66.4%). Bcl-2 overexpression was associated with the high expression of cyclin D1 (p=0.001). Correlation was detected between both cyclin D1 and bcl-2 and hormone receptor positivity (p<0.001). There was a reverse correlation between bcl-2 and histologic grade, p53, c-erbB2. And the bcl-2 overexpression group showed better disease free survival rates at 3-year follow up.

Conclusion

Higher expression of cyclin D1 was associated with bcl-2 overexpression. Positive estrogen receptor expression was associated with high cyclin D1 and bcl-2 expression. Bcl-2 tends to correlate with a positive clinical outcome.

Keywords: Bcl-2, Breast cancer, Cyclin D1, Prognosis

Figures and Tables

Fig 1

Immunohistochemical staining for cyclin D1 and Bcl-2. (A) Immunohistochemical nuclear staining of cyclin D1 expression of infiltrative ductal carcinoma (×200). (B) Immunohistochemical cytoplasmic staining of bcl-2 expression of infiltrative ductal carcinoma (×200).

Fig 2

Three year disease free survival with expression of cyclin D1 & bcl-2. (A) Three year disease free survival of cyclin D1 (p=0.632), (B) Three year disease free survival of bcl-2 (p=0.009).

Fig 3

Three year disease free survival with expression of cyclin D1 & bcl-2 in combination (p=0.061).

Table 1

General characteristics & clinicopathologic parameter of patients (n=342)

LN=lymph node; ER=estrogen receptor; PR=progesterone receptor.

Table 2

Correlation between clinicopathological factors and expression of cyclin D1, bcl-2 in infiltrative ductal carcinoma (n=342)

LN=lymph nodes; ER=estrogen receptor; PR=progesterone receptor.

Table 3

Cox multivariate analysis at 3 yr-disease free survival in invasive ductal carcinomas

ER=estrogen receptor; PR=progesterone receptor.

Table 4

Correlations between clinicopathological factors and expression of cyclin D1 and bcl-2 in combination

CD1=cyclin D1; LN=lymph nodes; ER=estrogen receptor; PR=progesterone receptor.

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